ArticleJournal of bacteriology2003
In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed.
- SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.Engineering in life sciences · 2025Article
- Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas.Microbial cell factories · 2024Article
- FleQ, FleN and c-di-GMP coordinately regulate cellulose production inFrontiers in molecular biosciences · 2023Article
- Article
- Synthesis of aromatic amino acids from 2G lignocellulosic substrates.Microbial biotechnology · 2021Article
- Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations.Applied microbiology and biotechnology · 2020Article
- Disappearance of Quorum Sensing in Burkholderia glumae During Experimental Evolution.Microbial ecology · 2020Article
- AmrZ and FleQ Co-regulate Cellulose Production inFrontiers in microbiology · 2019Article
- Molecular control of gene expression byThe Journal of biological chemistry · 2018Article
- The Activity of the Pseudomonas aeruginosa Virulence Regulator σ(VreI) Is Modulated by the Anti-σ Factor VreR and the Transcription Factor PhoB.Frontiers in microbiology · 2016Article
- Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering.Microbial cell factories · 2015Article
- Multiple signals modulate the activity of the complex sensor kinase TodS.Microbial biotechnology · 2015Article
- Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.Applied and environmental microbiology · 2014Article
- GtrS and GltR form a two-component system: the central role of 2-ketogluconate in the expression of exotoxin A and glucose catabolic enzymes in Pseudomonas aeruginosa.Nucleic acids research · 2014Article
- Identification of new residues involved in intramolecular signal transmission in a prokaryotic transcriptional repressor.Journal of bacteriology · 2014Article
- Transcriptional control by two interacting regulatory proteins: identification of the PtxS binding site at PtxR.Nucleic acids research · 2013Article
- The TetR family of regulators.Microbiology and molecular biology reviews : MMBR · 2013Review
- Characterization of BreR interaction with the bile response promoters breAB and breR in Vibrio cholerae.Journal of bacteriology · 2013Article
- Proteomic analysis of Pseudomonas putida reveals an organic solvent tolerance-related gene mmsB.PloS one · 2013Article
- Synthetic feedback loop model for increasing microbial biofuel production using a biosensor.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TtgGHI efflux pump of Pseudomonas putida DOT-T1E plays a key role in the innate and induced tolerance of this strain to aromatic hydrocarbons and antibiotics. The ttgGHI operon is expressed constitutively from two overlapping promoters in the absence of solvents and at a higher level in their presence, but not in response to antibiotics. Adjacent to the ttgGHI operon is the divergently transcribed ttgVW operon. In TtgV-deficient backgrounds, although not in a TtgW-deficient background, expression of the ttgGHI and ttgVW operons increased fourfold. This suggests that TtgV represses expression from the ttgG promoters and controls its own. TtgW plays no major role in the regulation of expression of these promoters. Primer extension revealed that the divergent ttgG and ttgV promoters overlap, and mobility shift assays indicated that TtgV binds to this region with high affinity. DNaseI footprint assays revealed that TtgV protected four DNA helical turns that include the -10 and -35 boxes of the ttgV and ttgG promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.