ArticleJournal of bacteriology2003
Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- Cellular and molecular outcomes of Pseudomonas putida KT2440 exposure to aluminium.Folia microbiologica · 2025Article
- Hypervesiculation Meets Sec-Targeting: Enhancing Heterologous Protein Loading inInternational journal of molecular sciences · 2025Article
- Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches.Microbiology spectrum · 2023Article
- Transcriptomic Response of the Diazotrophic BacteriaInternational journal of molecular sciences · 2022Article
- Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens.Molecular biology · 2021Article
- Comparative genomics of the transportome of Ten Treponema species.Microbial pathogenesis · 2019Article
- Article
- Deletion of a Gene Encoding a Putative Peptidoglycan-Associated Lipoprotein Prevents Degradation of the Crystalline Region of Cellulose inFrontiers in microbiology · 2018Article
- Defining the Roles of the Cation Diffusion Facilitators in FeFrontiers in cellular and infection microbiology · 2017Article
- Understanding butanol tolerance and assimilation in Pseudomonas putida BIRD-1: an integrated omics approach.Microbial biotechnology · 2016Article
- Genes Required for the Anti-fungal Activity of a Bacterial Endophyte Isolated from a Corn Landrace Grown Continuously by Subsistence Farmers Since 1000 BC.Frontiers in microbiology · 2016Article
- Identification of the Gene Cluster for the Anaerobic Degradation of 3,5-Dihydroxybenzoate (α-Resorcylate) in Thauera aromatica Strain AR-1.Applied and environmental microbiology · 2015Article
- Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex.Molecular microbiology · 2014Article
- Overexpression of the Escherichia coli TolQ protein leads to a null-FtsN-like division phenotype.MicrobiologyOpen · 2013Article
- High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation.The Journal of biological chemistry · 2013Article
- Lipoprotein biosynthesis as a target for anti-Wolbachia treatment of filarial nematodes.Parasites & vectors · 2010Article
- The caulobacter Tol-Pal complex is essential for outer membrane integrity and the positioning of a polar localization factor.Journal of bacteriology · 2010Article
- Regulation of glucose metabolism in Pseudomonas: the phosphorylative branch and entner-doudoroff enzymes are regulated by a repressor containing a sugar isomerase domain.The Journal of biological chemistry · 2009Article
- Comprehensive analyses of transport proteins encoded within the genome of "Aromatoleum aromaticum" strain EbN1.The Journal of membrane biology · 2009Article
- Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440.Microbial biotechnology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins of the Tol-Pal (Tol-OprL) system play a key role in the maintenance of outer membrane integrity and cell morphology in gram-negative bacteria. Here we describe an additional role for this system in the transport of various carbon sources across the cytoplasmic membrane. Growth of Pseudomonas putida tol-oprL mutant strains in minimal medium with glycerol, fructose, or arginine was impaired, and the growth rate with succinate, proline, or sucrose as the carbon source was lower than the growth rate of the parental strain. Assays with radiolabeled substrates revealed that the rates of uptake of these compounds by mutant cells were lower than the rates of uptake by the wild-type strain. The pattern and amount of outer membrane protein in the P. putida tol-oprL mutants were not changed, suggesting that the transport defect was not in the outer membrane. Consistently, the uptake of radiolabeled glucose and glycerol in spheroplasts was defective in the P. putida tol-oprL mutant strains, suggesting that there was a defect at the cytoplasmic membrane level. Generation of a proton motive force appeared to be unaffected in these mutants. To rule out the possibility that the uptake defect was due to a lack of specific transporter proteins, the PutP symporter was overproduced, but this overproduction did not enhance proline uptake in the tol-oprL mutants. These results suggest that the Tol-OprL system is necessary for appropriate functioning of certain uptake systems at the level of the cytoplasmic membrane.
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