ArticleJournal of bacteriology2003
Performance of standard phenotypic assays for TonB activity, as evaluated by varying the level of functional, wild-type TonB.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- The Intrinsically Disordered Region of ExbD Is Required for Signal Transduction.Journal of bacteriology · 2020Article
- Going Outside the TonB Box: Identification of Novel FepA-TonB InteractionsJournal of bacteriology · 2017Article
- Fluorescence High-Throughput Screening for Inhibitors of TonB Action.Journal of bacteriology · 2017Article
- ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest.Journal of bacteriology · 2013Article
- Overexpression of the Escherichia coli TolQ protein leads to a null-FtsN-like division phenotype.MicrobiologyOpen · 2013Article
- Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway.Journal of bacteriology · 2013Article
- The ExbD periplasmic domain contains distinct functional regions for two stages in TonB energization.Journal of bacteriology · 2012Article
- Mutations in the ExbB cytoplasmic carboxy terminus prevent energy-dependent interaction between the TonB and ExbD periplasmic domains.Journal of bacteriology · 2011Article
- Taking the Escherichia coli TonB transmembrane domain "offline"? Nonprotonatable Asn substitutes fully for TonB His20.Journal of bacteriology · 2011Article
- Article
- Cytoplasmic membrane protonmotive force energizes periplasmic interactions between ExbD and TonB.Molecular microbiology · 2009Article
- Interactions of the energy transducer TonB with noncognate energy-harvesting complexes.Journal of bacteriology · 2008Article
- Deletion and substitution analysis of the Escherichia coli TonB Q160 region.Journal of bacteriology · 2007Article
- His(20) provides the sole functionally significant side chain in the essential TonB transmembrane domain.Journal of bacteriology · 2007Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
- Import of the transfer RNase colicin D requires site-specific interaction with the energy-transducing protein TonB.Journal of bacteriology · 2005Article
- Free and hemophore-bound heme acquisitions through the outer membrane receptor HasR have different requirements for the TonB-ExbB-ExbD complex.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability of gram-negative bacterial cells to transport cobalamin and iron-siderophore complexes and their susceptibility to killing by some bacteriophages and colicins are characteristics routinely used to assay mutations of proteins in the TonB-dependent energy transduction system. These assays vary greatly in sensitivity and are subject to perturbation by overexpression of TonB and, perhaps, other proteins that contribute to the process. Thus, the choice of assay and the means by which a potential mutant is expressed can greatly influence the interpretation and recognition of a given mutant. In the present study, we expressed TonB at several different quantified levels in cells that were then subjected to a panel of assays. Our results suggest that it is reasonable to regard the assays as having windows of sensitivity. Thus, while no single assay satisfactorily spans the potential range of TonB activity, it is evident that certain assays are better suited for resolving small deviations from wild-type levels of activity, with others most useful when activity levels are very low. It is apparent from the results that the application of all possible assays to the characterization of new mutants will yield the most meaningful results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.