ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Evidence for chelatable zinc in the extracellular space of the hippocampus, but little evidence for synaptic release of Zn.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 169 citations in OpenAlex.
- Environmental Enrichment Engages Vesicular Zinc Signaling to Enhance Hippocampal Neurogenesis.Cells · 2023Article
- Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.Frontiers in nutrition · 2021Article
- General Aspects of Metal Ions as Signaling Agents in Health and Disease.Biomolecules · 2020Review
- Localization of Free and Bound Metal Species through X-Ray Synchrotron Fluorescence Microscopy in the Rodent Brain and Their Relation to Behavior.Brain sciences · 2019Review
- Modeling of Zinc Dynamics in the Synaptic Cleft: Implications for Cadherin Mediated Adhesion and Synaptic Plasticity.Frontiers in molecular neuroscience · 2018Article
- Zinc enhances hippocampal long-term potentiation at CA1 synapses through NR2B containing NMDA receptors.PloS one · 2018Article
- Modelling zinc changes at the hippocampal mossy fiber synaptic cleft.Journal of computational neuroscience · 2016Article
- Metalloneurochemistry and the Pierian Spring: 'Shallow Draughts Intoxicate the Brain'.Israel journal of chemistry · 2016Article
- Zinc Selectively Blocks Neurosteroid-Sensitive Extrasynaptic δGABAA Receptors in the Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Copper and protons directly activate the zinc-activated channel.Biochemical pharmacology · 2016Article
- The Free Zinc Concentration in the Synaptic Cleft of Artificial Glycinergic Synapses Rises to At least 1 μM.Frontiers in molecular neuroscience · 2016Article
- Modulation of extrasynaptic NMDA receptors by synaptic and tonic zinc.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slices.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Zn(II)-coordination modulated ligand photophysical processes - the development of fluorescent indicators for imaging biological Zn(II) ions.RSC advances · 2014Article
- Synaptic Zn2+ inhibits neurotransmitter release by promoting endocannabinoid synthesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Chelators for investigating zinc metalloneurochemistry.Current opinion in chemical biology · 2013Review
- Evidence for an extracellular zinc-veneer in rodent brains from experiments with Zn-ionophores and ZnT3 knockouts.ACS chemical neuroscience · 2012Article
- Synaptic release and extracellular actions of Zn2+ limit propagation of spreading depression and related events in vitro and in vivo.Journal of neurophysiology · 2012Article
- Vesicular zinc regulates the Ca2+ sensitivity of a subpopulation of presynaptic vesicles at hippocampal mossy fiber terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The neurophysiology and pathology of brain zinc.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Review
Corrections and comments
- Erratum issued
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Zinc colocalizes with glutamate in the synaptic vesicles of certain glutamatergic vesicles in the mammalian brain. Here, I introduce a method for detecting Zn in the extracellular space of brain slices and another method for detecting the passage of Zn out of the slice. In both cases, the fluorimetric Zn probe FluoZin-3 is used in conjunction with a slow Zn chelator, Ca-EDTA, to reduce background fluorescence. In addition, a new Zn chelator, ethylenediiminodi-2-pentanedioic acid, with little affinity for Ca or Mg is introduced. These tools are then used to show that little Zn (approximately 2 nm) is released during the course of synaptic transmission into the extracellular space. However, when hippocampal slices are subjected to a high potassium stimulus (50 mM) combined with an increase in osmolarity, Zn is externalized in the Timm's-stained areas (approximately 6 nm). This stimulus also leads to even greater Zn elevations in area CA1 that is only weakly stained by the Timm's method. Nevertheless, even under these conditions, little if any Zn makes its way out of the slices. I present evidence for a layer of Zn in the extracellular space that maps onto the Timm's stained region of the hippocampus. This Zn veneer appears to be loosely associated with molecules in the extracellular space and may be the raison d'être for vesicular Zn.
Indexed as
Identifiers
12890779PMC6740710W1890098172What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.