ArticleNucleic acids research2003
Development of a rapid, small-scale DNA repair assay for use on clinical samples.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Aging alters DNA structure-induced genetic instability in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Establishment of a Transformation CoupledFrontiers in oncology · 2020Article
- A rapid, safe, and quantitative in vitro assay for measurement of uracil-DNA glycosylase activity.Journal of molecular medicine (Berlin, Germany) · 2019Article
- Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells.Oncotarget · 2018Article
- Article
- Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins.Molecular cancer research : MCR · 2015Article
- Radiosensitisation of bladder cancer cells by panobinostat is modulated by Ku80 expression.Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology · 2013Article
- APOBEC3G enhances lymphoma cell radioresistance by promoting cytidine deaminase-dependent DNA repair.Blood · 2012Article
- Article
- Nonhomologous DNA end joining in cell-free extracts.Journal of nucleic acids · 2010Article
- Cyclin-C-dependent cell-cycle entry is required for activation of non-homologous end joining DNA repair in postmitotic neurons.Cell death and differentiation · 2010Article
- Non-homologous DNA end joining in normal and cancer cells and its dependence on break structures.Genetics and molecular biology · 2010Article
- In vitro non-homologous DNA end joining assays--the 20th anniversary.The international journal of biochemistry & cell biology · 2009Review
- Double-strand breaks repair in lymphoblastoid cell lines from sisters discordant for breast cancer from the New York site of the BCFR.Carcinogenesis · 2008Article
- Inhibition of double-strand break non-homologous end-joining by cisplatin adducts in human cell extracts.Nucleic acids research · 2005Article
- Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications.Nucleic acids research · 2004Article
- Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy.Antimicrobial agents and chemotherapy · 2004Article
- DNA double strand break repair in human bladder cancer is error prone and involves microhomology-associated end-joining.Nucleic acids research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double-strand breaks (DSBs) are the most lethal form of DNA damage. They can be repaired by one of two pathways, homologous recombination and non-homologous end joining (NHEJ). A NHEJ assay has previously been reported which measures joining using cell-free extracts and a linearised plasmid as DNA substrate. This assay was designed for 3 x 10(9) cells grown in vitro and utilised radioactively labelled substrate. We have scaled down the method to use smaller cell numbers in a variety of cell lines. Altering the cellular extraction procedure decreased background DNA contamination. The cleaner preparations allowed us to use SYBR Green I staining to identify joined products, which was as sensitive as 32P-end-labelled DNA. NHEJ was found in established tumour cell lines from different originating tissues, though actual levels and fidelity of repair differed. This method also allowed end joining to be assessed in clinical specimens (human blood, brain and bladder tumours) within 24 h of receiving samples. The application of this method will allow investigation of the role of DSB DNA repair pathways in human tumours.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.