ArticleNucleic acids research2003
Double-stranded RNA-mediated gene silencing in fission yeast.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2021Article
- Functional dissection of regulatory models using gene expression data of deletion mutants.PLoS genetics · 2013Article
- RNA interference with special reference to combating viruses of crustacea.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Hairpin dsRNA does not trigger RNA interference in Candida albicans cells.Yeast (Chichester, England) · 2011Article
- Armoring CRAds with p21/Waf-1 shRNAs: the next generation of oncolytic adenoviruses.Cancer gene therapy · 2010Article
- pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA - triggering in the mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2010Article
- Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA.The EMBO journal · 2009Article
- Gene discovery and transcript analyses in the corn smut pathogen Ustilago maydis: expressed sequence tag and genome sequence comparison.BMC genomics · 2007Article
- Targeted gene silencing in the model mushroom Coprinopsis cinerea (Coprinus cinereus) by expression of homologous hairpin RNAs.Eukaryotic cell · 2006Article
- Review
- Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with delta12-desaturase gene expression.Applied and environmental microbiology · 2005Article
- Open reading frames provide a rich pool of potential natural antisense transcripts in fungal genomes.Nucleic acids research · 2005Article
- A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe.Genes & development · 2004Article
- TbAGO1, an argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei.BMC biology · 2003Article
- RNA interference: biology, mechanism, and applications.Microbiology and molecular biology reviews : MMBR · 2003Review
- Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta).Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double-stranded RNA (dsRNA) can specifically inhibit gene expression in a variety of organisms by invoking post-transcriptional degradation of homologous mRNA. Here we show that dsRNA-mediated gene regulation also occurs in the fission yeast Schizosaccharomyces pombe. We present evidence that: (i) reporter gene silencing is significantly enhanced when additional non-coding sense RNA is co-expressed with antisense RNA; (ii) expression of a panhandle RNA also silences target gene expression; (iii) expression of dsRNA is associated with siRNAs; (iv) a novel host-encoded factor which enhances antisense RNA gene silencing also enhances panhandle RNA-mediated gene inhibition. Both the exogenously introduced lacZ and c-myc genes are shown to be susceptible to dsRNA- mediated gene silencing in this model. Taken together, these data indicate that RNA-mediated gene silencing can occur through a RNAi-like mechanism in fission yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.