ArticleBMC developmental biology2003
Reduced-folate carrier (RFC) is expressed in placenta and yolk sac, as well as in cells of the developing forebrain, hindbrain, neural tube, craniofacial region, eye, limb buds and heart.
Article in BMC developmental biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Development and Characterization of Curcumin Loaded PEGylated Niosomal Nanoparticles: Potential Anti-Cancer Effect on Breast Cancer Cells through RFC Gene Expression.Asian Pacific journal of cancer prevention : APJCP · 2025Article
- Diet Alters Micronutrient Pathways in the Gut and Placenta that Regulate Fetal Growth and Development in Pregnant Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Folic acid, one-carbon metabolism & childhood cancer.The Indian journal of medical research · 2017Review
- Mechanistic Target of Rapamycin Is a Novel Molecular Mechanism Linking Folate Availability and Cell Function.The Journal of nutrition · 2017Review
- Prevention of neural tube defects in Lrp2 mutant mouse embryos by folic acid supplementation.Birth defects research · 2017Article
- Evolutionary development of embryonic cerebrospinal fluid composition and regulation: an open research field with implications for brain development and function.Fluids and barriers of the CNS · 2016Review
- Increased synthesis of folate transporters regulates folate transport in conditions of ethanol exposure and folate deficiency.Molecular and cellular biochemistry · 2016Article
- Mono-2-ethylhexyl phthalate (MEHP) alters histiotrophic nutrition pathways and epigenetic processes in the developing conceptus.The Journal of nutritional biochemistry · 2016Article
- Genetic and epigenomic footprints of folate.Progress in molecular biology and translational science · 2012Review
- Mechanisms of membrane transport of folates into cells and across epithelia.Annual review of nutrition · 2011Review
- Article
- Identification of dynein light chain road block-1 as a novel interaction partner with the human reduced folate carrier.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Regulation of folate receptor 1 gene expression in the visceral endoderm.Birth defects research. Part A, Clinical and molecular teratology · 2009Article
- Membrane transporters and folate homeostasis: intestinal absorption and transport into systemic compartments and tissues.Expert reviews in molecular medicine · 2009Review
- Methods to evaluate the effect of ethanol on the folate analogue: fluorescein methotrexate uptake in human proximal tubular cells.Advances in pharmacological sciences · 2009Article
- Article
- The many flavors of hyperhomocyst(e)inemia: insights from transgenic and inhibitor-based mouse models of disrupted one-carbon metabolism.Antioxidants & redox signaling · 2007Review
- Expression of folate pathway genes in the cartilage of Hoxd4 and Hoxc8 transgenic mice.Birth defects research. Part A, Clinical and molecular teratology · 2006Article
- Homocysteine metabolism, hyperhomocysteinaemia and vascular disease: an overview.Journal of inherited metabolic disease · 2006Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundFolate is essential for cellular proliferation and tissue regeneration. As mammalian cells cannot synthesize folates de novo, tightly regulated cellular uptake processes have evolved to sustain sufficient levels of intracellular tetrahydrofolate cofactors to support biosynthesis of purines, pyrimidines, and some amino acids (serine, methionine). Though reduced-folate carrier (RFC) is one of the major proteins mediating folate transport, knowledge of the developmental expression of RFC is lacking. We utilized in situ hybridization and immunolocalization to determine the developmental distribution of RFC message and protein, respectively.
resultsIn the mouse, RFC transcripts and protein are expressed in the E10.0 placenta and yolk sac. In the E9.0 to E11.5 mouse embryo RFC is widely detectable, with intense signal localized to cell populations in the neural tube, craniofacial region, limb buds and heart. During early development, RFC is expressed throughout the eye, but by E12.5, RFC protein becomes localized to the retinal pigment epithelium (RPE).
conclusionsClinical studies show a statistical decrease in the number of neural tube defects, craniofacial abnormalities, cardiovascular defects and limb abnormalities detected in offspring of female patients given supplementary folate during pregnancy. The mechanism, however, by which folate supplementation ameliorates the occurrence of developmental defects is unclear. The present work demonstrates that RFC is present in placenta and yolk sac and provides the first evidence that it is expressed in the neural tube, craniofacial region, limb buds and heart during organogenesis. These findings suggest that rapidly dividing cells in the developing neural tube, craniofacial region, limb buds and heart may be particularly susceptible to folate deficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.