ArticleJournal of bioenergetics and biomembranes2003
Hydrogen peroxide- and cell-density-regulated expression of NADH-cytochrome b5 reductase in HeLa cells.
Article in Journal of bioenergetics and biomembranes, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
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- The Impact of Aging, Calorie Restriction and Dietary Fat on Autophagy Markers and Mitochondrial Ultrastructure and Dynamics in Mouse Skeletal Muscle.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- Levothyroxine enhances glucose clearance and blunts the onset of experimental type 1 diabetes mellitus in mice.British journal of pharmacology · 2017Article
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- Hydrogen Peroxide Is Involved inFrontiers in plant science · 2017Article
- Mitochondrial permeabilization without caspase activation mediates the increase of basal apoptosis in cells lacking Nrf2.Free radical biology & medicine · 2016Article
- Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2015Article
- Membrane-bound CYB5R3 is a common effector of nutritional and oxidative stress response through FOXO3a and Nrf2.Antioxidants & redox signaling · 2014Article
- Mitochondrial ultrastructure and markers of dynamics in hepatocytes from aged, calorie restricted mice fed with different dietary fats.Experimental gerontology · 2014Article
- Dietary fat modifies mitochondrial and plasma membrane apoptotic signaling in skeletal muscle of calorie-restricted mice.Age (Dordrecht, Netherlands) · 2013Article
- Alterations of ultrastructural and fission/fusion markers in hepatocyte mitochondria from mice following calorie restriction with different dietary fats.The journals of gerontology. Series A, Biological sciences and medical sciences · 2013Article
- Dietary oil modifies the plasma proteome during aging in the rat.Age (Dordrecht, Netherlands) · 2012Article
- Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues.Free radical biology & medicine · 2011Article
- Genetic deletion of Nrf2 promotes immortalization and decreases life span of murine embryonic fibroblasts.The journals of gerontology. Series A, Biological sciences and medical sciences · 2011Article
- Salmonella type III secretion effector SlrP is an E3 ubiquitin ligase for mammalian thioredoxin.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental conditions regulate the expression of different antioxidant enzymes in cell culture. We have studied the effect of cell density and hydrogen peroxide on the expression of NADH-cytochrome b5 reductase in HeLa cells. Polypeptide levels of the NADH-cytochrome b5 reductase increased about three fold in confluent HeLa cells compared to sparse cells. Addition of H2O2 to HeLa cells altered expression levels of the NADH-cytochrome b5 reducatase in a concentration-dependent way, being sparse cells more sensitive to H2O2 addition than confluent cells. The presence of pyruvate, a H2O2 scavenger, produced a significant increment (200%) in the levels of NADH-cytochrome b5 reductase in sparse cells, but less increase (25%) in confluent cells, suggesting that generation of endogenous H2O2 could repress NADH-cytochrome b5 reductase expression, particularly in sparse cultures. Accordingly, confluent HeLa cells showed significantly lower levels of reactive oxygen species than cells in sparse cultures. Addition of tert-butylhydroquinone, a compound which generates reactive oxygen species through redox cycling, also reduced expression of the NADH-cytochrome b5 reductase. Increments in several antioxidant enzymes taking place during confluency could participate in the increase of NADH-cytochrome b5 reductase expression by reducing reactive oxygen species levels in cells. Overall, our results support that acute oxidative stress caused by H2O2 inhibits the expression levels of NADH-cytochrome b5 reductase, most likely due to inhibition of SP1 transcriptional activity. On the other hand, adaptation to H2O2 involved increased expression of the cytochrome b5 reductase, supporting the existence of additional regulatory mechanisms.
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