ArticleJournal of virology2003
Selection of retroviral reverse transcription primer is coordinated with tRNA biogenesis.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed.
- Article
- Transcriptional and reverse transcriptional regulation of host genes by human endogenous retroviruses in cancers.Frontiers in microbiology · 2022Review
- Review
- Tie-Break: Host and Retrotransposons Play tRNA.Trends in cell biology · 2018Review
- tRNAs as primers and inhibitors of retrotransposons.Mobile genetic elements · 2017Review
- iPBS: a universal method for DNA fingerprinting and retrotransposon isolation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Forced selection of tRNA(Glu) reveals the importance of two adenosine-rich RNA loops within the U5-PBS for SIV(smmPBj) replication.Virology · 2007Article
- Nucleotides within the anticodon stem are important for optimal use of tRNA(Lys,3) as the primer for HIV-1 reverse transcription.Virology · 2007Article
- tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.Journal of virology · 2007Article
- Impact of forced selection of tRNAs on HIV-1 replication and genome stability highlight preferences for selection of certain tRNAs.Virus research · 2007Article
- Analysis of the replication of HIV-1 forced to use tRNAMet(i) supports a link between primer selection, translation and encapsidation.Retrovirology · 2007Article
- Importance of A-loop complementarity with tRNAHis anticodon for continued selection of tRNAHis as the HIV reverse transcription primer.Virology journal · 2007Article
- Complementation of human immunodeficiency virus type 1 replication by intracellular selection of Escherichia coli formula supplied in trans.Journal of virology · 2006Article
- Structural elements of the tRNA TPsiC loop critical for nucleocytoplasmic transport are important for human immunodeficiency virus type 1 primer selection.Journal of virology · 2005Article
- Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.Retrovirology · 2005Article
- tRNAs at the Virus-Host Interface.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Initiation of retrovirus reverse transcription requires the selection of a tRNA primer from the intracellular milieu. To investigate the features of primer selection, a human immunodeficiency virus type 1 (HIV-1) and a murine leukemia virus (MuLV) were created that require yeast tRNA(Phe) to be supplied in trans for infectivity. Wild-type yeast tRNA(Phe) expressed in mammalian cells was transported to the cytoplasm and aminoacylated. In contrast, tRNA(Phe) without the D loop (tRNA(Phe)D(-)) was retained within the nucleus and did not complement infectivity of either HIV-1 or MuLV; however, infectivity was restored when tRNA(Phe)D(-) was directly transfected into the cytoplasm of cells. A tRNA(Phe) mutant (tRNA(Phe)UUA) that did not have the capacity to be aminoacylated was transported to the cytoplasm and did complement infectivity of both HIV-1 and MuLV, albeit at a level less than that with wild-type tRNA(Phe). Collectively, our results demonstrate that the tRNA primer captured by HIV-1 and MuLV occurs after nuclear export of tRNA and supports a model in which primer selection for retroviruses is coordinated with tRNA biogenesis at the intracellular site of protein synthesis.
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