ArticleJournal of virology2003
Identification of a region of the tobacco mosaic virus 126- and 183-kilodalton replication proteins which binds specifically to the viral 3'-terminal tRNA-like structure.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- Diagnosis and Characterization of Plant Viruses Using HTS to Support Virus Management and Tomato Breeding.Viruses · 2024Article
- The non-template functions of helper virus RNAs create optimal replication conditions to enhance the proliferation of satellite RNAs.PLoS pathogens · 2024Article
- Receptor-like kinase BAM1 facilitates early movement of the Tobacco mosaic virus.Communications biology · 2021Article
- Structures and Functions of the 3' Untranslated Regions of Positive-Sense Single-Stranded RNA Viruses Infecting Humans and Animals.Frontiers in cellular and infection microbiology · 2020Review
- Plant Viruses in Plant Molecular Pharming: Toward the Use of Enveloped Viruses.Frontiers in plant science · 2019Review
- TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3'UTR affecting viral RNAs accumulation and symptom expression.Scientific reports · 2015Article
- Proof by synthesis of Tobacco mosaic virus.Genome biology · 2014Article
- Replication protein of tobacco mosaic virus cotranslationally binds the 5' untranslated region of genomic RNA to enable viral replication.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro.Journal of virology · 2012Article
- The dependence of viral RNA replication on co-opted host factors.Nature reviews. Microbiology · 2011Review
- Modification of small RNAs associated with suppression of RNA silencing by tobamovirus replicase protein.Journal of virology · 2007Article
- Identification of a ribonucleoprotein intermediate of tomato mosaic virus RNA replication complex formation.Journal of virology · 2007Article
- In vitro synthesis of minus-strand RNA by an isolated cereal yellow dwarf virus RNA-dependent RNA polymerase requires VPg and a stem-loop structure at the 3' end of the virus RNA.Journal of virology · 2006Article
- Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound.Journal of virology · 2006Article
- Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
UV irradiation of a mixture of an isolated tobacco mosaic virus (TMV; tomato strain L [TMV-L]) RNA-dependent RNA polymerase complex and the TMV-L RNA 3'-terminal region (3'-TR) resulted in cross-linking of the TMV-L 126-kDa replication protein to the TMV-L 3'-TR. Using both Escherichia coli-expressed proteins corresponding to parts of the 126-kDa protein and mutants of the 3'-TR, the interacting sites were located to a 110-amino-acid region just downstream of the core methyltransferase domain in the protein and a region comprising the central core C and domain D2 in the 3'-TR. Mutation to alanine of a tyrosine residue at position 409 or a tyrosine residue at position 416 in the protein binding region abolished cross-linking to the 3'-TR, and corresponding mutations introduced into TMV-L RNA abolished its ability to replicate in tomato protoplasts, with no detectable production of either plus- or minus-strand RNA. The results are compatible with a model for initiation of TMV-L minus-strand RNA synthesis in which an internal region of the TMV-L 126-kDa protein first binds to the central core C and domain D2 region of the TMV-L 3'-TR and is then followed by binding of the 183-kDa protein to this complex and positioning of the catalytically active site of the polymerase domain close to the 3'-terminal CCCA initiation site.
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