ArticleBiophysical journal2003
Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- From cells to pixels: A decision tree for designing bioimage analysis pipelines.Journal of microscopy · 2025Article
- Computational analysis of treatment resistant cancer cells.Frontiers in cell and developmental biology · 2025Article
- Modulation of the cytoskeleton for cancer therapy.Frontiers in cell and developmental biology · 2025Article
- BNP-Track: A framework for multi-particle superresolved tracking.bioRxiv : the preprint server for biology · 2023Article
- Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Diatrack particle tracking software: Review of applications and performance evaluation.Traffic (Copenhagen, Denmark) · 2017Review
- Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The FAK-Arp2/3 interaction promotes leading edge advance and haptosensing by coupling nascent adhesions to lamellipodia actin.Molecular biology of the cell · 2016Article
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
- Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics.Biophysical journal · 2014Article
- Visualizing cytoplasmic flow during single-cell wound healing in Stentor coeruleus.Journal of visualized experiments : JoVE · 2013Article
- Arp2/3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow.The Journal of cell biology · 2012Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Myosin-1A targets to microvilli using multiple membrane binding motifs in the tail homology 1 (TH1) domain.The Journal of biological chemistry · 2012Article
- The role of actin turnover in retrograde actin network flow in neuronal growth cones.PloS one · 2012Article
- Optimal-Flow Minimum-Cost Correspondence Assignment in Particle Flow Tracking.Computer vision and image understanding : CVIU · 2011Article
- Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules.Molecular biology of the cell · 2010Article
- Single mRNA tracking in live cells.Methods in enzymology · 2010Article
- Computational image analysis of cellular dynamics: a case study based on particle tracking.Cold Spring Harbor protocols · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fluorescent speckle microscopy (FSM) is becoming the technique of choice for analyzing in vivo the dynamics of polymer assemblies, such as the cytoskeleton. The massive amount of data produced by this method calls for computational approaches to recover the quantities of interest; namely, the polymerization and depolymerization activities and the motions undergone by the cytoskeleton over time. Attempts toward this goal have been hampered by the limited signal-to-noise ratio of typical FSM data, by the constant appearance and disappearance of speckles due to polymer turnover, and by the presence of flow singularities characteristic of many cytoskeletal polymer assemblies. To deal with these problems, we present a particle-based method for tracking fluorescent speckles in time-lapse FSM image series, based on ideas from operational research and graph theory. Our software delivers the displacements of thousands of speckles between consecutive frames, taking into account that speckles may appear and disappear. In this article we exploit this information to recover the speckle flow field. First, the software is tested on synthetic data to validate our methods. We then apply it to mapping filamentous actin retrograde flow at the front edge of migrating newt lung epithelial cells. Our results confirm findings from previously published kymograph analyses and manual tracking of such FSM data and illustrate the power of automated tracking for generating complete and quantitative flow measurements. Third, we analyze microtubule poleward flux in mitotic metaphase spindles assembled in Xenopus egg extracts, bringing new insight into the dynamics of microtubule assemblies in this system.
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