ArticleBiophysical journal2003
Myosin head configuration in relaxed insect flight muscle: x-ray modeled resting cross-bridges in a pre-powerstroke state are poised for actin binding.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 77 citations in OpenAlex.
- Fine-tuning striated muscle performance: conserved sarcomere-level mechanisms across insect and vertebrate systems.Frontiers in physiology · 2026Review
- Nanometer scale difference in myofilament lattice structure of muscle alters muscle function in a spatially explicit model.PLoS computational biology · 2025Article
- John Squire and the myosin thick filament structure in muscle.Journal of muscle research and cell motility · 2023Review
- Small Angle X-ray Diffraction as a Tool for Structural Characterization of Muscle Disease.International journal of molecular sciences · 2022Review
- Analysis methods and quality criteria for investigating muscle physiology using x-ray diffraction.The Journal of general physiology · 2021Article
- Article
- An insight on Drosophila myogenesis and its assessment techniques.Molecular biology reports · 2020Review
- Special Issue: The Actin-Myosin Interaction in Muscle: Background and Overview.International journal of molecular sciences · 2019Review
- Article
- Monitoring the myosin crossbridge cycle in contracting muscle: steps towards 'Muscle-the Movie'.Journal of muscle research and cell motility · 2019Review
- Through thick and thin: dual regulation of insect flight muscle and cardiac muscle compared.Journal of muscle research and cell motility · 2019Review
- Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.International journal of molecular sciences · 2019Review
- Structure of myosin filaments from relaxedScience advances · 2016Article
- Dynamics of cross-bridge cycling, ATP hydrolysis, force generation, and deformation in cardiac muscle.Journal of molecular and cellular cardiology · 2016Article
- Myosin S2 origins track evolution of strong binding on actin by azimuthal rolling of motor domain.Biophysical journal · 2015Article
- Three-dimensional structure of the human myosin thick filament: clinical implications.Global cardiology science & practice · 2013Review
- Early events in helix unfolding under external forces: a milestoning analysis.The journal of physical chemistry. B · 2012Article
- The function of the M-line protein obscurin in controlling the symmetry of the sarcomere in the flight muscle of Drosophila.Journal of cell science · 2012Article
- Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.PloS one · 2012Article
- Head-head interactions of resting myosin crossbridges in intact frog skeletal muscles, revealed by synchrotron x-ray fiber diffraction.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Low-angle x-ray diffraction patterns from relaxed insect flight muscle recorded on the BioCAT beamline at the Argonne APS have been modeled to 6.5 nm resolution (R-factor 9.7%, 65 reflections) using the known myosin head atomic coordinates, a hinge between the motor (catalytic) domain and the light chain-binding (neck) region (lever arm), together with a simulated annealing procedure. The best head conformation angles around the hinge gave a head shape that was close to that typical of relaxed M*ADP*Pi heads, a head shape never before demonstrated in intact muscle. The best packing constrained the eight heads per crown within a compact crown shelf projecting at approximately 90 degrees to the filament axis. The two heads of each myosin molecule assume nonequivalent positions, one head projecting outward while the other curves round the thick filament surface to nose against the proximal neck of the projecting head of the neighboring molecule. The projecting heads immediately suggest a possible cross-bridge cycle. The relaxed projecting head, oriented almost as needed for actin attachment, will attach, then release Pi followed by ADP, as the lever arm with a purely axial change in tilt drives approximately 10 nm of actin filament sliding on the way to the nucleotide-free limit of its working stroke. The overall arrangement appears well designed to support precision cycling for the myogenic oscillatory mode of contraction with its enhanced stretch-activation response used in flight by insects equipped with asynchronous fibrillar flight muscles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.