ArticleBiophysical journal2003
Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.Biochimica et biophysica acta. Biomembranes · 2026Article
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.bioRxiv : the preprint server for biology · 2026Article
- Do Nanodisc Assembly Conditions Affect Natural Lipid Uptake?Journal of the American Society for Mass Spectrometry · 2023Article
- Model Plasma Membrane Exhibits a Microemulsion in Both Leaves Providing a Foundation for "Rafts".Biophysical journal · 2020Article
- Lipid Structure and Composition Control Consequences of Interleaflet Coupling in Asymmetric Vesicles.Biophysical journal · 2018Article
- The Chemical Potential of Plasma Membrane Cholesterol: Implications for Cell Biology.Biophysical journal · 2018Article
- Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins.Scientific reports · 2017Article
- Phosphatidylserine Lateral Organization Influences the Interaction of Influenza Virus Matrix Protein 1 with Lipid Membranes.Journal of virology · 2017Article
- A dual-mediated liposomal drug delivery system targeting the brain: rational construction, integrity evaluation across the blood-brain barrier, and the transporting mechanism to glioma cells.International journal of nanomedicine · 2017Article
- Atomic Recombination in Dynamic Secondary Ion Mass Spectrometry Probes Distance in Lipid Assemblies: A Nanometer Chemical Ruler.Journal of the American Chemical Society · 2016Article
- Phase diagram of a polyunsaturated lipid mixture: Brain sphingomyelin/1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine/cholesterol.Biochimica et biophysica acta · 2016Article
- On scattered waves and lipid domains: detecting membrane rafts with X-rays and neutrons.Soft matter · 2015Article
- Elastic Membrane Deformations Govern Interleaflet Coupling of Lipid-Ordered Domains.Physical review letters · 2015Article
- Computer simulations suggest a key role of membranous nanodomains in biliary lipid secretion.PLoS computational biology · 2015Article
- Selective association of outer surface lipoproteins with the lipid rafts of Borrelia burgdorferi.mBio · 2014Article
- Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.Biochimica et biophysica acta · 2013Article
- Phase behavior and domain size in sphingomyelin-containing lipid bilayers.Biochimica et biophysica acta · 2013Article
- Measurement of lipid nanodomain (raft) formation and size in sphingomyelin/POPC/cholesterol vesicles shows TX-100 and transmembrane helices increase domain size by coalescing preexisting nanodomains but do not induce domain formation.Biophysical journal · 2011Article
- Phase separation in lipid membranes.Cold Spring Harbor perspectives in biology · 2011Review
- Development of fluorophore dynamics imaging as a probe for lipid domains in model vesicles and cell membranes.European biophysics journal : EBJ · 2011Article
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Abstract
An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differing in their affinities for ordered versus disordered regions of the bilayer, and an acceptor lipid that distributes preferentially into disordered regions. Inhomogeneities in bilayer organization, on spatial scales of tens of nanometers or greater, are detected as a marked difference in the efficiencies of quenching of fluorescence of the two donor species by the acceptor. Using a novel pair of 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD)-labeled tetraacyl lipids as donor species with a rhodaminyl-labeled acceptor, this strategy faithfully reports homo- versus inhomogeneous mixing in each of several lipid bilayer systems whose organization on the FRET distance scale can be predicted from previous findings. Interestingly, however, the present FRET method reports clear evidence of inhomogeneity in the organization of mixtures combining sphingomyelin or saturated phospholipids with unsaturated phospholipids and physiological proportions of cholesterol, even at physiological temperatures where these systems have been reported to appear homogeneous by fluorescence microscopy. These results indicate that under physiological conditions, lipid mixtures mimicking the lipid composition of the outer leaflet of the plasma membrane can form domains on a spatial scale comparable to that inferred for the dimensions of lipid rafts in biological membranes.
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