ArticleBiophysical journal2003
CymA of Klebsiella oxytoca outer membrane: binding of cyclodextrins and study of the current noise of the open channel.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- How to isolate channel-forming membrane proteins using the E. coli expression system.Nature protocols · 2025Review
- Conformational flexibility driving charge-selective substrate translocation across a bacterial transporter.Chemical science · 2024Article
- Cyclodextrins: Only Pharmaceutical Excipients or Full-Fledged Drug Candidates?Pharmaceutics · 2022Review
- The C2 entity of chitosugars is crucial in molecular selectivity of the Vibrio campbellii chitoporin.The Journal of biological chemistry · 2021Article
- Role of Electroosmosis in the Permeation of Neutral Molecules: CymA and Cyclodextrin as an Example.Biophysical journal · 2016Article
- Chitoporin from the Marine Bacterium Vibrio harveyi: PROBING THE ESSENTIAL ROLES OF TRP136 AT THE SURFACE OF THE CONSTRICTION ZONE.The Journal of biological chemistry · 2015Article
- Outer-membrane translocation of bulky small molecules by passive diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Protein reconstitution into freestanding planar lipid membranes for electrophysiological characterization.Nature protocols · 2015Article
- Enhanced antibacterial and anti-quorum sensing activities of triclosan by complexation with modified β-cyclodextrins.World journal of microbiology & biotechnology · 2013Article
- Peptide translocation through the mesoscopic channel: binding kinetics at the single molecule level.European biophysics journal : EBJ · 2013Article
- Chitoporin from Vibrio harveyi, a channel with exceptional sugar specificity.The Journal of biological chemistry · 2013Article
- Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Anthrax toxin protective antigen: inhibition of channel function by chloroquine and related compounds and study of binding kinetics using the current noise analysis.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CymA, the outer membrane component of the cyclodextrin (CD) uptake and metabolism system of Klebsiella oxytoca, was reconstituted into lipid bilayer membranes. The channel properties of this unusual porin were studied in detail. The binding of CDs to the channel resulted in its complete block for ion transport. This result allowed the detailed investigation of carbohydrate binding, and the stability constants for the binding of cyclic and linear carbohydrates to the binding site inside the channel were calculated from titration experiments of the membrane conductance with the carbohydrates. Highest stability constant was observed for alpha-cyclodextrin (alpha-CD; K = 32,000 1/M) followed by beta-cyclodextrin (beta-CD; K = 1970 1/M) and gamma-cyclodextrin (gamma-CD; K = 310 1/M). Linear maltooligosaccharides bound also to CymA but with much smaller stability constants as compared to cyclic ones. The noise of the current through CymA in multi- and single-channel experiments was investigated using fast Fourier transformation. The current through the open channels had a rather high spectral density, which was a Lorentzian function of the frequency up to 2000 Hz. Upon addition of cyclic dextrins to the aqueous phase the spectral density decreased in a dose-dependent manner, which made it impossible to evaluate the binding kinetics. Experiments with single CymA-channels demonstrated the channel is highly asymmetric concerning channel flickers and current noise.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.