ArticleTransgenic research2003
The endosperm and the embryo of Arabidopsis thaliana are independently transformed through infiltration by Agrobacterium tumefaciens.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis.The New phytologist · 2026Article
- Tissue culture-independent approaches to revolutionizing plant transformation and gene editing.Horticulture research · 2025Article
- Identification of theInternational journal of molecular sciences · 2020Article
- The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study.Molecular biology reports · 2020Review
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Cysteine-rich receptor-like kinase CRK5 as a regulator of growth, development, and ultraviolet radiation responses in Arabidopsis thaliana.Journal of experimental botany · 2015Article
- TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.Frontiers in plant science · 2015Article
- Overexpression of the poplar NF-YB7 transcription factor confers drought tolerance and improves water-use efficiency in Arabidopsis.Journal of experimental botany · 2013Article
- Application of HB17, an Arabidopsis class II homeodomain-leucine zipper transcription factor, to regulate chloroplast number and photosynthetic capacity.Journal of experimental botany · 2013Article
- BBX32, an Arabidopsis B-Box protein, functions in light signaling by suppressing HY5-regulated gene expression and interacting with STH2/BBX21.Plant physiology · 2011Article
- Article
- Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Chimeric proteins suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their specific sites in the cellulose synthase of primary walls.Plant physiology · 2006Article
- Agrobacterium T-DNA integration in Arabidopsis is correlated with DNA sequence compositions that occur frequently in gene promoter regions.Functional & integrative genomics · 2005Article
- High-frequency gene targeting in Arabidopsis plants expressing the yeast RAD54 gene.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development.Genes & development · 2004Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several experiments had indicated that in planta transformation of Arabidopsis thaliana by Agrobacterium involves the female germ line. In order to identify the precise stage at which transformation occurs we have monitored expression of a gusA reporter gene in the two products of the double fertilization of infiltrated plants. The plantlets and the remaining endosperm of seeds were separately tested after germination. It appeared that in the majority of cases only the plantlet or the endosperm were transformed. Based on transformation with two vectors borne by two different Agrobacterium strains, the minority of 'co-transformed' plantlets and endosperm can be explained by simultaneous but independent transformation events. These results indicate that mature female gametes could be the targets of T-DNA.
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