ArticleTransgenic research2003
Transgene organisation in potato after particle bombardment-mediated (co-)transformation using plasmids and gene cassettes.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Development of a facile genetic transformation system for the Spanish elite rice paella genotype Bomba.Transgenic research · 2022Article
- State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential.Frontiers in plant science · 2021Review
- Introduction of Arabidopsis's heat shock factor HsfA1d mitigates adverse effects of heat stress on potato (Solanum tuberosum L.) plant.Cell stress & chaperones · 2020Article
- The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat.Transgenic research · 2015Article
- Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants.Transgenic research · 2013Article
- Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator.Plant cell reports · 2011Article
- Generation of marker-free Bt transgenic indica rice and evaluation of its yellow stem borer resistance.Journal of applied genetics · 2010Article
- Marker-free transgenic corn plant production through co-bombardment.Plant cell reports · 2009Article
- Article
- Co-integration, co-expression and inheritance of unlinked minimal transgene expression cassettes in an apomictic turf and forage grass (Paspalum notatum Flugge).Plant cell reports · 2008Article
- Cytokinin vectors mediate marker-free and backbone-free plant transformation.Transgenic research · 2008Article
- Efficient and stable transformation of hop (Humulus lupulus L.) var. Eroica by particle bombardment.Plant cell reports · 2008Article
- Optimization of wheat co-transformation procedure with gene cassettes resulted in an improvement in transformation frequency.Molecular biology reports · 2007Article
- Transgene constructs in coho salmon (Oncorhynchus kisutch) are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite DNA.Transgenic research · 2006Article
- In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research.Plant methods · 2006Article
- Biolistic transformation of grapevine using minimal gene cassette technology.Plant cell reports · 2006Article
- Direct gene transfer in potato: a comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts.Plant cell reports · 2005Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protocols for efficient co-transformation of potato internodes with genes contained in separate plasmids or gene cassettes (i.e., linear PCR fragments comprising a promoter-gene-terminator) using particle bombardment were established. Twenty-eight out of 62 (45%) and 11 out of 65 (17%) plants transformed with a plasmid containing the selectable marker contained one and two additional non-selected genes, respectively. When gene cassettes were used in transformation, six out of eight plants were co-transformed. Expression analysis showed that 75-80% of the plants transformed with two transgenes expressed both of them, irrespective of the use of plasmids or gene cassettes. Thirty-eight plants containing the gusA reporter-gene and the nptII selectable-marker have been characterised with respect to the molecular organisation of the donor DNAs. Seventeen out of 49 (35%) gusA sites of integration contained one copy of the gene. Only 11 gusA sites (22%) were linked to the site of integration of the selectable marker. When one site of integration contained several copies of the transgene, a predominance of 3'-3' inverted re-arrangement repeats was observed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.