ArticleTransgenic research2003
Homology-dependent DNA transfer from plants to a soil bacterium under laboratory conditions: implications in evolution and horizontal gene transfer.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering.FEMS microbiology ecology · 2026Article
- Plastid engineering using episomal DNA.Plant cell reports · 2023Review
- Survival and DNA Damage in Plant Seeds Exposed for 558 and 682 Days outside the International Space Station.Astrobiology · 2017Article
- Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples.Transgenic research · 2010Article
- Sexual isolation in Acinetobacter baylyi is locus-specific and varies 10,000-fold over the genome.Genetics · 2009Article
- Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics.Transgenic research · 2007Review
- Horizontal transfer of a plant transposon.PLoS biology · 2006Article
- Risk mitigation of genetically modified bacteria and plants designed for bioremediation.Journal of industrial microbiology & biotechnology · 2005Review
- Will transgenic plants adversely affect the environment?Journal of biosciences · 2005Review
- Unsuccessful search for DNA transfer from transgenic plants to bacteria in the intestine of the tobacco horn worm, Manduca sexta.Transgenic research · 2005Article
- Health considerations regarding horizontal transfer of microbial transgenes present in genetically modified crops.Journal of biomedicine & biotechnology · 2005Article
- Problems in monitoring horizontal gene transfer in field trials of transgenic plants.Nature biotechnology · 2004Article
- Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase.Plant molecular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA transfer was demonstrated from six species of donor plants to the soil bacterium, Acinetobacter spp. BD413, using neomycin phosphotransferase (nptII) as a marker for homologous recombination. These laboratory results are compatible with, but do not prove, DNA transfer in nature. In tobacco carrying a plastid insertion of nptII, transfer was detected with 0.1 g of disrupted leaves and in oilseed rape carrying a nuclear insertion with a similar quantity of roots. Transfer from disrupted leaves occurred in sterile soil and water, without the addition of nutrients. It was detected using intact tobacco leaves and intact tobacco and Arabidopsis plants in vitro. Transfer was dose-dependent and sensitive to DNase, and mutations in the plant nptII were recovered in receptor bacteria. DNA transfer using intact roots and plants in vitro was easily demonstrated, but with greater variability. Transfer varied with plant genome size and the number of repeats of the marker DNA in the donor plant. Transfer was not detected in the absence of a homologous nptII in the receptor bacteria. We discuss these results with reference to non-coding DNA in plant genomes (e.g., introns, transposons and junk DNA) and the possibility that DNA transfer could occur in nature.
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Registered trials
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