ArticleMethods in cell biology2003
Live-cell imaging of slow axonal transport in cultured neurons.
Article in Methods in cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Imaging Intracellular Trafficking in Neurons of C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A possible mechanism for neurofilament slowing down in myelinated axon: Phosphorylation-induced variation of NF kinetics.PloS one · 2021Article
- In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration Using Manganese-Enhanced MRI.Methods in molecular biology (Clifton, N.J.) · 2018Article
- KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons.Traffic (Copenhagen, Denmark) · 2016Article
- Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.Neurochemical research · 2016Article
- Giant axonal neuropathy-associated gigaxonin mutations impair intermediate filament protein degradation.The Journal of clinical investigation · 2013Article
- Object tracking with particle filtering in fluorescence microscopy images: application to the motion of neurofilaments in axons.IEEE transactions on medical imaging · 2012Article
- The role of stretching in slow axonal transport.Biophysical journal · 2011Article
- Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons.Cell motility and the cytoskeleton · 2007Article
- Neurofilaments switch between distinct mobile and stationary states during their transport along axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Neurofilament polymer transport in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Arrival, reversal, and departure of neurofilaments at the tips of growing axons.Molecular biology of the cell · 2004Article
- The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Cytoskeletal polymers and other cytosolic protein complexes are transported along axons in the slow components of axonal transport. Studies on the movement of neurofilaments and microtubules in the axons of cultured neurons indicate that these polymers actually move at fast rates and that the movements are also infrequent and highly asynchronous. These observations indicate that the slow rate of slow axonal transport is due to rapid movements interrupted by prolonged pauses which presents special challenges for studies on the mechanism of movement. This chapter describes the procedures that the author's laboratory has used to observe and analyze the movement of neurofilaments and microtubules in axons of cultured neurons from the superior cervical ganglia of neonatal rats. In particular, the author describes how to culture these neurons, how to transfect them by nuclear injection, and how to detect the rapid and infrequent movement of cytoskeletal polymers using time-lapse fluorescence imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.