ArticleMedical microbiology and immunology2004
Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR.
Article in Medical microbiology and immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using theFrontiers in microbiology · 2024Pooled it
- A rare case of lepromatous leprosy in Germany.Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG · 2025Article
- Quantitative Real-Time Polymerase Chain Reaction for Detection of Mycobacterium leprae DNA in Tissue Specimens from Patients with Leprosy.The American journal of tropical medicine and hygiene · 2023Article
- Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis.PLoS neglected tropical diseases · 2022Article
- Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study.PLoS neglected tropical diseases · 2019Article
- Article
- Non-exponential growth of Mycobacterium leprae Thai-53 strain cultured in vitro.Microbiology and immunology · 2016Article
- Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae.Journal of microbiology (Seoul, Korea) · 2015Article
- PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic.PLoS neglected tropical diseases · 2014Review
- Application of RLEP real-time PCR for detection of M. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy.The American journal of tropical medicine and hygiene · 2014Article
- Use of the polymerase chain reaction to detect Mycobacterium leprae in urine.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2012Article
- Orientia tsutsugamushi bacteremia and cytokine levels in Vietnamese scrub typhus patients.Journal of clinical microbiology · 2009Article
- Enumeration of Mycobacterium leprae using real-time PCR.PLoS neglected tropical diseases · 2008Article
- Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy.Journal of clinical microbiology · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare test.Journal of clinical microbiology · 2005Article
- qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.