ArticleThe Journal of physiology2003
Lucifer Yellow slows voltage-gated Na+ current inactivation in a light-dependent manner in mice.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Slow recovery from the inactivation of voltage-gated sodium channel Nav1.3 in mouse taste receptor cells.Pflugers Archiv : European journal of physiology · 2021Article
- Cell-type-independent expression of inwardly rectifying potassium currents in mouse fungiform taste bud cells.Physiological research · 2020Article
- Reactive species modify NaV1.8 channels and affect action potentials in murine dorsal root ganglion neurons.Pflugers Archiv : European journal of physiology · 2016Article
- Sodium channels as gateable non-photonic sensors for membrane-delimited reactive species.Biochimica et biophysica acta · 2014Article
- Retrograde fluorescent labeling allows for targeted extracellular single-unit recording from identified neurons in vivo.Journal of visualized experiments : JoVE · 2013Article
- Dual somatic recordings from gonadotropin-releasing hormone (GnRH) neurons identified by green fluorescent protein (GFP) in hypothalamic slices.Journal of visualized experiments : JoVE · 2010Article
- Oxidation of multiple methionine residues impairs rapid sodium channel inactivation.Pflugers Archiv : European journal of physiology · 2008Article
- Functional expression of ionotropic purinergic receptors on mouse taste bud cells.The Journal of physiology · 2007Article
- Integration time in a subset of spinal lamina I neurons is lengthened by sodium and calcium currents acting synergistically to prolong subthreshold depolarization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Physiological, neurochemical and morphological properties of a subgroup of GABAergic spinal lamina II neurones identified by expression of green fluorescent protein in mice.The Journal of physiology · 2004Article
- Citrate transport in the human prostate epithelial PNT2-C2 cell line: electrophysiological analyses.The Journal of physiology · 2004Article
- Distinctive membrane and discharge properties of rat spinal lamina I projection neurones in vitro.The Journal of physiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lucifer Yellow CH (LY), a membrane-impermeant fluorescent dye, has been used in electro-physiological studies to visualize cell morphology, with little concern about its pharmacological effects. We investigated its effects on TTX-sensitive voltage-gated Na+ channels in mouse taste bud cells and hippocampal neurons under voltage-damp conditions. LY applied inside cells irreversibly slowed the inactivation of Na+ currents upon exposure to light of usual intensities. The inactivation time constant of Na+ currents elicited by a depolarization to -15 mV was increased by fourfold after a 5 min exposure to halogen light of 3200 Ix at source (3200 Ix light), and sevenfold after a 1-min exposure to 12,000 Ix light. A fraction of the Na+ current became non-inactivating following the exposure. The non-inactivating current was approximately 20 % of the peak total Na+ current after a 5 min exposure to 3200 Ix light, and approximately 30 % after a 1 min exposure to 12,000 Ix light. Light-exposed LY shifted slightly the current-voltage relationship of the peak Na+ current and of the steady-state inactivation curve, in the depolarizing direction. A similar light-dependent decrease in kinetics occurred in whole-cell Na+ currents of cultured mouse hippocampal neurones. Single-channel recordings showed that exposure to 6500 Ix light for 3 min increased the mean open time of Na+channels from 1.4 ms to 2.4 ms without changing the elementary conductance. The pre-incubation of taste bud cells with 1 mM dithiothreitoL a scavenger of radical species, blocked these LY effects. These results suggest that light-exposed LY yields radical species that modify Na+ channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.