ArticleMarine biotechnology (New York, N.Y.)
RecA-mediated, targeted mutagenesis in zebrafish.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Precision editing of large animal genomes.Advances in genetics · 2012Review
- Use of RecA fusion proteins to induce genomic modifications in zebrafish.Nucleic acids research · 2011Article
- Photobiological effects of UVA and UVB light in zebrafish embryos: evidence for a competent photorepair system.Journal of photochemistry and photobiology. B, Biology · 2007Article
- Homologous recombination in zebrafish ES cells.Transgenic research · 2006Article
- Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis.Nucleic acids research · 2005Article
- Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish.Transgenic research · 2004Article
- Replacement of buried cysteine from zebrafish Cu/Zn superoxide dismutase and enhancement of its stability via site-directed mutagenesis.Marine biotechnology (New York, N.Y.)Article
- Site-directed gene integration in transgenic zebrafish mediated by cre recombinase using a combination of mutant lox sites.Marine biotechnology (New York, N.Y.)Article
- Efficient transfection strategy for the spatiotemporal control of gene expression in zebrafish.Marine biotechnology (New York, N.Y.)Article
- Integration of double-fluorescence expression vectors into zebrafish genome for the selection of site-directed knockout/knockin.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have evaluated the efficacy of RecA, a prokaryotic protein involved with homologous recombination, to direct site-specific mutagenesis in zebrafish embryos. For this we coinjected a vector containing a mutated enhanced green fluorescent protein (EGFP) gene plus 236-nucleotide corrective single-stranded DNAs coated with RecA into 1-cell zebrafish embryos. Twenty-hours after fertilization, about 5% to 20% of injected embryos showed EGFP expression in 1 or more cells when RecA-coated corrective DNAs were used, but not when RecA was omitted. Mutated EGFP genes with 1-bp insertions or deletions were inefficiently activated, whereas those with 7-bp insertions were activated about 4-fold more efficiently. RecA-coated template strand had a higher efficiency than its complementary strand in activation of EGFP expression. Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.