ArticleInfection and immunity2003
Functional selection of vaccine candidate peptides from Staphylococcus aureus whole-genome expression libraries in vitro.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
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- Cell-free Biosynthesis of Peptidomimetics.Biotechnology and bioprocess engineering : BBE · 2023Review
- Biosynthesis of titanium dioxide nanoparticles using Hypericum perforatum and Origanum vulgare extracts and their main components, hypericin and carvacrol as promising antibacterial agents.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2022Article
- Vaccines and Immunoinformatics for Vaccine Design.Advances in experimental medicine and biology · 2022Article
- Advanced strategies for development of vaccines against human bacterial pathogens.World journal of microbiology & biotechnology · 2021Review
- Application of median lethal concentration (LCBMC research notes · 2020Article
- Nucleic Acid-Barcoding Technologies: Converting DNA Sequencing into a Broad-Spectrum Molecular Counter.Angewandte Chemie (International ed. in English) · 2019Review
- PanRV: Pangenome-reverse vaccinology approach for identifications of potential vaccine candidates in microbial pangenome.BMC bioinformatics · 2019Article
- Selection of single-chain variable fragments specific forIranian journal of basic medical sciences · 2017Article
- Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis.Microbial cell factories · 2015Article
- Biofilm matrix exoproteins induce a protective immune response against Staphylococcus aureus biofilm infection.Infection and immunity · 2014Article
- Study of the inhibitory effect of Quercus Coccifera's aqueous extract on Staphylococcus aureus and Pseudomonas aeruginosa In vitro.Bioinformation · 2014Article
- Cloning, Expression and Purification of Penicillin Binding Protein2a (PBP2a) from Methicillin Resistant Staphylococcus aureus: A Study on Immunoreactivity in Balb/C Mouse.Avicenna journal of medical biotechnology · 2013Article
- Immunorelevant proteins for the diagnosis of bovine staphylococcal mastitis.World journal of microbiology & biotechnology · 2013Article
- CD4 T cell antigens from Staphylococcus aureus Newman strain identified following immunization with heat-killed bacteria.Clinical and vaccine immunology : CVI · 2012Article
- Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli.BMC microbiology · 2011Article
- Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis.Veterinary research · 2011Article
- Functional selection of hepatitis C virus envelope E2-binding Peptide ligands by using ribosome display.Antimicrobial agents and chemotherapy · 2010Article
- Proteomics-based identification of anchorless cell wall proteins as vaccine candidates against Staphylococcus aureus.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro protein selection method, ribosome display, has been applied to comprehensively identify and map the immunologically relevant proteins of the human pathogen Staphylococcus aureus. A library built up from genomic fragments of the virulent S. aureus COL strain (methicillin-resistant S. aureus) allowed us to screen all possible encoded peptides for immunoreactivity. As selective agents, human sera exhibiting a high antibody titer and opsonic activity against S. aureus were used, since these antibodies indicate the in vivo expression and immunoreactivity of the corresponding proteins. Identified clones cluster in distinct regions of 75 genes, most of them classifiable as secreted or surface-localized proteins, including previously identified virulence factors. In addition, 14 putative novel short open reading frames were identified and their immunoreactivity and in vivo mRNA expression were confirmed, underscoring the annotation-independent, true genomic nature of our approach. Evidence is provided that a large fraction of the identified peptides cannot be expressed in an in vivo-based surface display system. Thus, in vitro protein selection, not biased by the context of living entities, allows screening of genomic expression libraries with a large number of different ligands simultaneously. It is a powerful approach for fingerprinting the repertoire of immune reactive proteins serving as target candidates for active and passive vaccination against pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.