ArticleInfection and immunity2003
Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 108 citations in OpenAlex.
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- Identification of Cross Reactive Antigens of C. botulinum Types A, B, E & F by Immunoproteomic Approach.Current microbiology · 2018Article
- Next-Generation Bacillus anthracis Live Attenuated Spore Vaccine Based on the htrA(-) (High Temperature Requirement A) Sterne Strain.Scientific reports · 2016Article
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- Identification of the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R.PloS one · 2013Article
- Investigating the genome diversity of B. cereus and evolutionary aspects of B. anthracis emergence.Genomics · 2011Article
- Curing the plasmid pXO2 from Bacillus anthracis A16 using plasmid incompatibility.Current microbiology · 2011Article
- Immunospecific responses to bacterial elongation factor Tu during Burkholderia infection and immunization.PloS one · 2010Article
- Comparative proteome analysis of Bacillus anthracis with pXO1 plasmid content.Journal of microbiology (Seoul, Korea) · 2010Article
- A novel strategy of epitope design in Neisseria gonorrhoeae.Bioinformation · 2010Article
- Novel and unique diagnostic biomarkers for Bacillus anthracis infection.Applied and environmental microbiology · 2009Article
- Vaccinology in the genome era.The Journal of clinical investigation · 2009Review
- Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3.BMC microbiology · 2009Article
- Identification of Bacillus anthracis spore component antigens conserved across diverse Bacillus cereus sensu lato strains.Molecular & cellular proteomics : MCP · 2009Article
- The antigenome: from protein subunit vaccines to antibody treatments of bacterial infections?Advances in experimental medicine and biology · 2009Review
- Identification and experimental verification of protective antigens against Streptococcus suis serotype 2 based on genome sequence analysis.Current microbiology · 2009Article
- Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome.Infection and immunity · 2007Article
- Cytosolic proteins contribute to surface plasminogen recruitment of Neisseria meningitidis.Journal of bacteriology · 2007Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis (Ames strain) chromosome-derived open reading frames (ORFs), predicted to code for surface exposed or virulence related proteins, were selected as B. anthracis-specific vaccine candidates by a multistep computational screen of the entire draft chromosome sequence (February 2001 version, 460 contigs, The Institute for Genomic Research, Rockville, Md.). The selection procedure combined preliminary annotation (sequence similarity searches and domain assignments), prediction of cellular localization, taxonomical and functional screen and additional filtering criteria (size, number of paralogs). The reductive strategy, combined with manual curation, resulted in selection of 240 candidate ORFs encoding proteins with putative known function, as well as 280 proteins of unknown function. Proteomic analysis of two-dimensional gels of a B. anthracis membrane fraction, verified the expression of some gene products. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses allowed identification of 38 spots cross-reacting with sera from B. anthracis immunized animals. These spots were found to represent eight in vivo immunogens, comprising of EA1, Sap, and 6 proteins whose expression and immunogenicity was not reported before. Five of these 8 immunogens were preselected by the bioinformatic analysis (EA1, Sap, 2 novel SLH proteins and peroxiredoxin/AhpC), as vaccine candidates. This study demonstrates that a combination of the bioinformatic and proteomic strategies may be useful in promoting the development of next generation anthrax vaccine.
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