ArticleInfection and immunity2003
Characterization of new members of the group 3 outer membrane protein family of Brucella spp.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Application of Outer Membrane Protein Omp25 in Serological Diagnosis of Brucellosis.Infectious disease reports · 2026Review
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- Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.Frontiers in microbiology · 2024Article
- Article
- A sensor histidine kinase from a plant-endosymbiont bacterium restores the virulence of a mammalian intracellular pathogen.Microbial pathogenesis · 2023Article
- Cross regulation in a three-component cell envelope stress signaling system ofbioRxiv : the preprint server for biology · 2023Article
- An ArsR Transcriptional Regulator FacilitatesInternational journal of molecular sciences · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 ofFrontiers in cellular and infection microbiology · 2020Article
- Comparison of Immune Effects BetweenFrontiers in veterinary science · 2020Article
- MapB, the Brucella suis TamB homologue, is involved in cell envelope biogenesis, cell division and virulence.Scientific reports · 2019Article
- Alterations of microRNAs and their predicted targeting mRNAs expression in RAW264.7 macrophages infected with Omp25 mutant Brucella melitensis.Innate immunity · 2018Article
- Characterization of Cell Envelope Multiple Mutants ofFrontiers in microbiology · 2018Article
- Article
- In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b.Clinical and experimental vaccine research · 2016Article
- Brucella outer membrane protein Omp25 induces microglial cells in vitro to secrete inflammatory cytokines and inhibit apoptosis.International journal of clinical and experimental medicine · 2015Article
- Analyzing the molecular mechanism of lipoprotein localization in Brucella.Frontiers in microbiology · 2015Review
- Evaluation of the efficacy of outer membrane protein 31 vaccine formulations for protection against Brucella canis in BALB/c mice.Clinical and vaccine immunology : CVI · 2014Article
- Evaluation in mice of Brucella ovis attenuated mutants for use as live vaccines against B. ovis infection.Veterinary research · 2014Article
- 16S rRNA and omp31 gene based molecular characterization of field strains of B. melitensis from aborted foetus of goats in India.TheScientificWorldJournal · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Impairment of the omp25 gene in Brucella spp. leads to attenuated strains and confers protection to the host. Omp25 and Omp31, whose functions remain unknown, were the first characterized members of group 3 outer membrane proteins (Omps) (25 to 34 kDa). Recently, genomic and proteomic approaches identified five new putative members of this family, some of which are produced in B. melitensis or B. abortus. In the present study, using protein microsequencing, we identified new members of group 3 Omps proteins produced in B. suis. Since several monoclonal antibodies (MAbs) against Omp25 cross-reacted with other members of group 3 Omps, we also performed Western immunoblotting to compare wild-type B. suis with mutants systematically having B. suis omp25-related genes knocked out. We demonstrate the production of three paralogs of Omp31 and/or Omp25 in B. suis, and the existence of a common site of signal peptide cleavage (AXAAD), which is very similar to that present in the five homologous Omps of Bartonella quintana. The seven group 3 Omps were classified in four-subgroups on the basis of percentage amino acid sequence identities: Omp25 alone, the Omp25b-Omp25c-Omp25d cluster, the Omp31/31b subgroup, and the less related Omp22 protein (also called Omp3b). Together with previous data, our results demonstrate that all new members of group 3 Omps are produced in B. suis or in other Brucella species and we propose a nomenclature that integrates all of these proteins to facilitate the understanding of future Brucella interspecies study results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.