ArticleProceedings of the National Academy of Sciences of the United States of America2003
Cloning and characterization of the Drosophila U7 small nuclear RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 29 citations in OpenAlex.
- U7 snRNA: A tool for gene therapy.The journal of gene medicine · 2021Review
- Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery.Methods in enzymology · 2021Article
- Composition and processing activity of a semi-recombinant holo U7 snRNP.Nucleic acids research · 2020Article
- Protein composition of catalytically active U7-dependent processing complexes assembled on histone pre-mRNA containing biotin and a photo-cleavable linker.Nucleic acids research · 2018Article
- A subset of replication-dependent histone mRNAs are expressed as polyadenylated RNAs in terminally differentiated tissues.Nucleic acids research · 2016Article
- Non-coding RNAs, the cutting edge of histone messages.RNA biology · 2016Article
- RIP-seq analysis of eukaryotic Sm proteins identifies three major categories of Sm-containing ribonucleoproteins.Genome biology · 2014Article
- Functional analysis of the integrator subunit 12 identifies a microdomain that mediates activation of the Drosophila integrator complex.The Journal of biological chemistry · 2013Article
- A subset of Drosophila integrator proteins is essential for efficient U7 snRNA and spliceosomal snRNA 3'-end formation.Molecular and cellular biology · 2011Article
- Article
- The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are required for histone pre-mRNA processing and play an essential role in development.RNA (New York, N.Y.) · 2009Article
- U7 snRNAs: a computational survey.Genomics, proteomics & bioinformatics · 2007Article
- Review
- The Drosophila melanogaster Cajal body.The Journal of cell biology · 2006Article
- U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis.RNA (New York, N.Y.) · 2006Article
- Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray.Nucleic acids research · 2006Article
- Organization of the Caenorhabditis elegans small non-coding transcriptome: genomic features, biogenesis, and expression.Genome research · 2006Article
- Differences and similarities between Drosophila and mammalian 3' end processing of histone pre-mRNAs.RNA (New York, N.Y.) · 2005Article
- Evolutionary patterns of non-coding RNAs.Theory in biosciences = Theorie in den Biowissenschaften · 2005Article
- A CPSF-73 homologue is required for cell cycle progression but not cell growth and interacts with a protein having features of CPSF-100.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Base pairing between the 5' end of U7 small nuclear RNA (snRNA) and the histone downstream element (HDE) in replication-dependent histone pre-mRNAs is the key event in 3'-end processing that leads to generation of mature histone mRNAs. We have cloned the Drosophila U7 snRNA and demonstrated that it is required for histone pre-mRNA 3'-end processing in a Drosophila nuclear extract. The 71-nt Drosophila U7 snRNA is encoded by a single gene that is embedded in the direct orientation in an intron of the Eip63E gene. The U7 snRNA gene contains conserved promoter elements typical of other Drosophila snRNA genes, and the coding sequence is followed by a 3' box indicating that the Drosophila U7 snRNA gene is an independent transcription unit. Drosophila U7 snRNA contains a trimethyl-guanosine cap at the 5' end and a putative Sm-binding site similar to the unique Sm-binding site found in other U7 snRNAs. Drosophila U7 snRNA is approximately 10 nt longer than mammalian U7 snRNAs because of an extended 5' sequence and has only a limited potential to form a stem-loop structure near the 3' end. The extended 5' end of Drosophila U7 snRNA can base pair with the HDE in all five Drosophila histone pre-mRNAs. Blocking the 5' end of the U7 snRNA with a complementary oligonucleotide specifically blocks processing of a Drosophila histone pre-mRNA. Changes in the HDE that abolish or decrease processing efficiency result in a reduced ability to recruit U7 snRNA to the pre-mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.