ArticleMolecular and cellular biochemistry2003
Poly(ADPR) polymerase-1 and poly(ADPR) glycohydrolase level and distribution in differentiating rat germinal cells.
Article in Molecular and cellular biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- ADP-ribose hydrolases: biological functions and potential therapeutic targets.Expert reviews in molecular medicine · 2024Review
- Multi-targeted Effect of Nicotinamide Mononucleotide on Brain Bioenergetic Metabolism.Neurochemical research · 2019Review
- CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation.Neurochemical research · 2017Article
- The PARP family: insights into functional aspects of poly (ADP-ribose) polymerase-1 in cell growth and survival.Cell proliferation · 2016Review
- Association of common SNP rs1136410 in PARP1 gene with the susceptibility to male infertility with oligospermia.Journal of assisted reproduction and genetics · 2014Article
- X-ray induced DNA damage and repair in germ cells of PARP1(-/-) male mice.International journal of molecular sciences · 2013Article
- Macro domains as metabolite sensors on chromatin.Cellular and molecular life sciences : CMLS · 2013Review
- Role of poly(ADP-ribose) polymerases in male reproduction.Spermatogenesis · 2013Article
- Potential biological role of poly (ADP-ribose) polymerase (PARP) in male gametes.Reproductive biology and endocrinology : RB&E · 2009Review
- The role of NAD biosynthesis in plant development and stress responses.Annals of botany · 2009Review
- Mono-galloyl glucose derivatives are potent poly(ADP-ribose) glycohydrolase (PARG) inhibitors and partially reduce PARP-1-dependent cell death.British journal of pharmacology · 2008Article
- Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?Microbiology and molecular biology reviews : MMBR · 2006Review
- Poly(ADP-ribose) glycohydrolase silencing protects against H2O2-induced cell death.The Biochemical journal · 2006Article
- Article
- Depletion of the 110-kilodalton isoform of poly(ADP-ribose) glycohydrolase increases sensitivity to genotoxic and endotoxic stress in mice.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly(ADP-ribose)polymerase (PARP-1) and poly(ADP-ribose)glycohydrolase (PARG) are responsible for the transient poly(ADP-ribosyl)ation of proteins in eukaryotic cells. This biochemical reaction plays an active role in DNA replication and repair, transcription, cell differentiation and death. The aim of this study was to investigate the levels and the sub-cellular distribution of such enzymes in rat germinal cells at different stages of differentiation, i.e. in primary spermatocytes and round spermatids, representing meiotic and post-meiotic cells, respectively. The determination of the level of PARP-1 mRNA and protein revealed its higher expression in primary spermatocytes, thus implying that PARP-1 is one of the meiotic genes whose expression is requested at the pachytene phase of the meiosis. We also demonstrated that rat germinal cells contain both the forms of PARG (i.e. of 110 and 60 kDa) so far described in somatic cells. In our experimental system, the large PARG was present and active mainly in the nuclear fraction of primary spermatocytes, whereas round spermatids showed a higher level of the 60 kDa PARG in the post-nuclear fraction. Collectively, our data show a different expression level of PARP-1 and a different endocellular distribution of PARG and suggest a role for the poly(ADP-ribose) turnover in distinct pathways in meiotic and post-meiotic germinal cells.
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