Evidence map›Paper›PMID 12869703›Full record

ArticleRNA (New York, N.Y.)2003

Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec.

Mahadev Rao, Bradley A Carlson, Sergey V Novoselov, Donald P Weeks, Vadim N Gladyshev, Dolph L Hatfield

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 34 citations in OpenAlex.

  1. Review
  2. Review
  3. Selenocysteine tRNAMethods in molecular biology (Clifton, N.J.) · 2018
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Genomics of Volvocine Algae.Advances in botanical research
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mahadev RaoSection on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Bradley A Carlson
Sergey V Novoselov
Donald P Weeks
Vadim N Gladyshev
Dolph L Hatfield
National Institutes of Health · USUniversity of Nebraska–Lincoln · US

Funding

IDENTITY OF TERMINATOR AND SELENOCYSTEINE UGA CODONSR01GM061603 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GLADYSHEV, VADIM N. · 2000 to 2016
$4.0M
Role of Selenium in Development and HealthZIABC005317 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI HATFIELD, DOLPH · 2009 to 2014
$2.7M
ROLE OF SELENIUM IN PREVENTING CANCER, HEART DISEASE AND DELAYING AGING PROCESSZ01BC005317 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI HATFIELD, DOLPH · 1996 to 2008
$1.5M
NIGMS NIH HHS GM061603NIGMS NIH HHS R01 GM061603
6 · The paper itself

Abstract

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec tRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

Indexed as

AnimalsBase SequenceChlamydomonas reinhardtiiEvolution, MolecularMolecular Sequence DataNucleic Acid ConformationRNA, Transfer, Amino AcylSequence Homology, Nucleic AcidRNA, Transfer, Amino Acylselenocysteinyl-tRNA

Identifiers

PMID12869703
PMCPMC1370458
OpenAlexW2147092581

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.