ArticleThe Journal of biological chemistry2003
Expression cloning and biochemical characterization of a Rhizobium leguminosarum lipid A 1-phosphatase.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Outer membrane engineering through lipid A modification allows modulation ofInfection and immunity · 2026Article
- Lipid a remodeling modulates outer membrane vesicle biogenesis byJournal of bacteriology · 2025Article
- Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.Cell reports · 2022Article
- Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates withiScience · 2021Article
- TheMicroorganisms · 2021Article
- Metabolic engineering of Escherichia coli to produce a monophosphoryl lipid A adjuvant.Metabolic engineering · 2020Article
- Aminosugar-based immunomodulator lipid A: synthetic approaches.Beilstein journal of organic chemistry · 2018Review
- Identification ofFrontiers in microbiology · 2017Article
- Bacterial strategies of resistance to antimicrobial peptides.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Kdo2 -lipid A: structural diversity and impact on immunopharmacology.Biological reviews of the Cambridge Philosophical Society · 2015Review
- Stress-induced outer membrane vesicle production by Pseudomonas aeruginosa.Journal of bacteriology · 2013Article
- Fortifying the barrier: the impact of lipid A remodelling on bacterial pathogenesis.Nature reviews. Microbiology · 2013Review
- Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.Functional & integrative genomics · 2013Article
- Article
- The calcium-stimulated lipid A 3-O deacylase from Rhizobium etli is not essential for plant nodulation.Biochimica et biophysica acta · 2012Article
- Analysis of the networks controlling the antimicrobial-peptide-dependent induction of Klebsiella pneumoniae virulence factors.Infection and immunity · 2011Article
- Three phosphatidylglycerol-phosphate phosphatases in the inner membrane of Escherichia coli.The Journal of biological chemistry · 2011Article
- Escherichia coli mutants that synthesize dephosphorylated lipid A molecules.Biochemistry · 2010Article
- Altered lipid A structures and polymyxin hypersensitivity of Rhizobium etli mutants lacking the LpxE and LpxF phosphatases.Biochimica et biophysica acta · 2010Article
- Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1- and 4'-phosphatase activities.Cellular microbiology · 2009Article
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Lipid A of Rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, displays several significant structural differences when compared with Escherichia coli. An especially striking feature of R. leguminosarum lipid A is that it lacks both the 1- and 4'-phosphate groups. Distinct lipid A phosphatases that attack either the 1 or the 4' positions have previously been identified in extracts of R. leguminosarum and Rhizobium etli but not Sinorhizobium meliloti or E. coli. Here we describe the identification of a hybrid cosmid (pMJK-1) containing a 25-kb R. leguminosarum 3841 DNA insert that directs the overexpression of the lipid A 1-phosphatase. Transfer of pMJK-1 into S. meliloti 1021 results in heterologous expression of 1-phosphatase activity, which is normally absent in extracts of strain 1021, and confers resistance to polymyxin. Sequencing of a 7-kb DNA fragment derived from the insert of pMJK-1 revealed the presence of a lipid phosphatase ortholog (designated LpxE). Expression of lpxE in E. coli behind the T7lac promoter results in the appearance of robust 1-phosphatase activity, which is normally absent in E. coli membranes. Matrix-assisted laser-desorption/time of flight and radiochemical analysis of the product generated in vitro from the model substrate lipid IVA confirms the selective removal of the 1-phosphate group. These findings show that lpxE is the structural gene for the 1-phosphatase. The availability of lpxE may facilitate the re-engineering of lipid A structures in diverse Gram-negative bacteria and allow assessment of the role of the 1-phosphatase in R. leguminosarum symbiosis with plants. Possible orthologs of LpxE are present in some intracellular human pathogens, including Francisella tularensis, Brucella melitensis, and Legionella pneumophila.
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