ArticleThe Biochemical journal2003
Interferon-gamma regulates nucleoside transport systems in macrophages through signal transduction and activator of transduction factor 1 (STAT1)-dependent and -independent signalling pathways.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Nucleoside transporters and immunosuppressive adenosine signaling in the tumor microenvironment: Potential therapeutic opportunities.Pharmacology & therapeutics · 2022Review
- Single-Molecule RNA Sequencing Reveals IFNγ-Induced Differential Expression of Immune Escape Genes in Merkel Cell Polyomavirus-Positive MCC Cell Lines.Frontiers in microbiology · 2021Article
- mRNA Expression and Activity of Nucleoside Transporters in Human Hepatoma HepaRG Cells.Pharmaceutics · 2018Article
- Emerging Roles of Nucleoside Transporters.Frontiers in pharmacology · 2018Article
- Modulators of the Balance between M1 and M2 Macrophages during Pregnancy.Frontiers in immunology · 2017Review
- Nuclear Localization of Suppressor of Cytokine Signaling-1 Regulates Local Immunity in the Lung.Frontiers in immunology · 2016Article
- Modulators of Macrophage Polarization Influence Healing of the Infarcted Myocardium.International journal of molecular sciences · 2015Review
- Interferon gamma induces protective non-canonical signaling pathways in primary neurons.Journal of neurochemistry · 2015Article
- Transmembrane protein 106a activates mouse peritoneal macrophages via the MAPK and NF-κB signaling pathways.Scientific reports · 2015Article
- The probiotic mixture VSL#3 dampens LPS-induced chemokine expression in human dendritic cells by inhibition of STAT-1 phosphorylation.PloS one · 2014Article
- Molecular pathways regulating macrophage polarization: implications for atherosclerosis.Current atherosclerosis reports · 2012Review
- CNT1 expression influences proliferation and chemosensitivity in drug-resistant pancreatic cancer cells.Cancer research · 2011Article
- Identification and characterization of proximal promoter polymorphisms in the human concentrative nucleoside transporter 2 (SLC28A2).The Journal of pharmacology and experimental therapeutics · 2009Article
- Subtype-specific regulation of equilibrative nucleoside transporters by protein kinase CK2.The Biochemical journal · 2005Article
- Nucleoside transporters in chronic lymphocytic leukaemia.Leukemia · 2004Review
- KCNE gene expression is dependent on the proliferation and mode of activation of leukocytes.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expressions of CNT and ENT (concentrative and equilibrative nucleoside transporters) in macrophages are differentially regulated by IFN-gamma (interferon-gamma). This cytokine controls gene expression through STAT1-dependent and/or -independent pathways (where STAT1 stands for signal transduction and activator of transcription 1). In the present study, the role of STAT1 in the response of nucleoside transporters to IFN-gamma was studied using macrophages from STAT1 knockout mice. IFN-gamma triggered an inhibition of ENT1-related nucleoside transport activity through STAT1-dependent mechanisms. Such inhibition of macrophage growth and ENT1 activity by IFN-gamma is required for DNA synthesis. Interestingly, IFN-gamma led to an induction of the CNT1- and CNT2-related nucleoside transport activities independent of STAT1, thus ensuring the supply of extracellular nucleosides for the STAT1-independent RNA synthesis. IFN-gamma up-regulated CNT2 mRNA and CNT1 protein levels and down-regulated ENT1 mRNA in both wild-type and STAT1 knockout macrophages. This is consistent with a STAT1-independent, long-term-mediated, probably transcription-dependent, regulation of nucleoside transporter genes. Moreover, STAT1-dependent post-transcriptional mechanisms are implicated in the regulation of ENT1 activity. Although nitric oxide is involved in the regulation of ENT1 activity in B-cells at a post-transcriptional level, our results show that STAT1-dependent induction of nitric oxide by IFN-gamma is not implicated in the regulation of ENT1 activity in macrophages. Our results indicate that both STAT1-dependent and -independent pathways are involved in the regulation of nucleoside transporters by IFN-gamma in macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.