ArticleJournal of bacteriology2003
Cell death in Pseudomonas aeruginosa biofilm development.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 258 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
258 citing papers in PubMed, 581 citations in OpenAlex.
- Chemical prophage induction selectively removes Vibrio from a pelagic Sargassum-derived multispecies biofilm.Environmental microbiome · 2026Article
- A novel stress response pathway mediates biofilm architecture in Pseudomonas aeruginosa.PLoS pathogens · 2026Article
- Disrupting phage liquid crystalline droplets restores antibiotic susceptibility in Pseudomonas aeruginosa biofilms.PLoS biology · 2026Article
- Single Treatment of Mature 3D Single-, Dual- and Poly-Species Biofilms Using a Combination Therapy of Phage or Phage-Hetero-Cocktails and Ciprofloxacin.Antibiotics (Basel, Switzerland) · 2026Article
- Probing biofilm development, stress response and heterogeneity-spectroscopic characterization of single and multi-species consortia.NPJ biofilms and microbiomes · 2026Article
- A nutrient bottleneck controls antibiotic efficacy in structured bacterial populations.Nature communications · 2026Article
- Mutations in filamentous bacteriophages spark eco-evolutionary feedbacks in Pseudomonas aeruginosa.The ISME journal · 2026Article
- High-resolution visualization of biofilm matrix development in space and time using fluorescent stains for cellulose.NPJ biofilms and microbiomes · 2026Article
- Co-infection withISME communications · 2026Article
- Temperate bacteriophage induced inBiofilm · 2025Article
- Dispersal Mechanisms in Biofilm Control: Characteristics, Induction, Impacts, and Therapeutic Potential.Current microbiology · 2025Review
- Single-cell lysis patterns morphogenesis of eDNA in the matrix ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biofilm-associated molecular patterns: BAMPs.Infection and immunity · 2025Review
- Cell differentiation, aging, and death in spatially organized yeast communities: mechanisms and consequences.Cell death and differentiation · 2025Review
- Crosstalk between inovirus core gene and accessory toxin-antitoxin system mediates polylysogeny.Nature communications · 2025Article
- Targeted disruption of phage liquid crystalline droplets abolishes antibiotic tolerance of bacterial biofilms.bioRxiv : the preprint server for biology · 2025Article
- New Pseudomonas infections drive Pf phage transmission in CF airways.JCI insight · 2025Article
- Disruption ofMicrobiology (Reading, England) · 2025Article
- Article
- Real-time high-resolution microscopy reveals how single-cell lysis shapes biofilm matrix morphogenesis.bioRxiv : the preprint server for biology · 2024Article
198 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria growing in biofilms often develop multicellular, three-dimensional structures known as microcolonies. Complex differentiation within biofilms of Pseudomonas aeruginosa occurs, leading to the creation of voids inside microcolonies and to the dispersal of cells from within these voids. However, key developmental processes regulating these events are poorly understood. A normal component of multicellular development is cell death. Here we report that a repeatable pattern of cell death and lysis occurs in biofilms of P. aeruginosa during the normal course of development. Cell death occurred with temporal and spatial organization within biofilms, inside microcolonies, when the biofilms were allowed to develop in continuous-culture flow cells. A subpopulation of viable cells was always observed in these regions. During the onset of biofilm killing and during biofilm development thereafter, a bacteriophage capable of superinfecting and lysing the P. aeruginosa parent strain was detected in the fluid effluent from the biofilm. The bacteriophage implicated in biofilm killing was closely related to the filamentous phage Pf1 and existed as a prophage within the genome of P. aeruginosa. We propose that prophage-mediated cell death is an important mechanism of differentiation inside microcolonies that facilitates dispersal of a subpopulation of surviving cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.