ArticleJournal of bacteriology2003
Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters.PLoS pathogens · 2023Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Miniature Seed6, encoding an endoplasmic reticulum signal peptidase, is critical in seed development.Plant physiology · 2021Article
- Article
- Temporal proteomic profiling reveals changes that support Burkholderia biofilms.Pathogens and disease · 2019Article
- Secretome of obligate intracellular Rickettsia.FEMS microbiology reviews · 2015Review
- Rickettsia typhi possesses phospholipase A2 enzymes that are involved in infection of host cells.PLoS pathogens · 2013Article
- TolC-dependent secretion of an ankyrin repeat-containing protein of Rickettsia typhi.Journal of bacteriology · 2012Article
- Functional characterization of a phospholipase A(2) homolog from Rickettsia typhi.Journal of bacteriology · 2010Article
- Characterization of Sec-translocon-dependent extracytoplasmic proteins of Rickettsia typhi.Journal of bacteriology · 2008Article
- Genome-wide screen for temperature-regulated genes of the obligate intracellular bacterium, Rickettsia typhi.BMC microbiology · 2008Article
- Additional in vitro and in vivo evidence for SecA functioning as dimers in the membrane: dissociation into monomers is not essential for protein translocation in Escherichia coli.Journal of bacteriology · 2008Article
- The lspA gene, encoding the type II signal peptidase of Rickettsia typhi: transcriptional and functional analysis.Journal of bacteriology · 2007Article
- A universally applicable method of operon map prediction on minimally annotated genomes using conserved genomic context.Nucleic acids research · 2005Article
- Isolation and characterization of type I signal peptidase of different malaria parasites.Journal of biomedicine & biotechnology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 nucleotides, respectively. Alignment analysis of the deduced amino acid sequences reveals the presence of highly conserved motifs that are important for the catalytic activity of bacterial type I signal peptidase. Reverse transcription-PCR and Northern blot analysis demonstrated that the lepB gene of R. rickettsii is cotranscribed in a polycistronic message with the putative nuoF (encoding NADH dehydrogenase I chain F), secF (encoding protein export membrane protein), and rnc (encoding RNase III) genes in a secF-nuoF-lepB-rnc cluster. The cloned lepB genes from R. rickettsii and R. typhi have been demonstrated to possess signal peptidase I activity in Escherichia coli preprotein processing in vivo by complementation assay.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.