ArticleJournal of bacteriology2003
IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lactococcus lactis.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 78 citations in OpenAlex.
- Tailored molecular weight hyaluronic acid production by engineered Lactococcus lactis.Microbial cell factories · 2026Article
- Experimental evolution under different nutritional conditions changes the genomic architecture and virulence of Acinetobacter baumannii.Communications biology · 2024Article
- Natural diversity of lactococci in γ-aminobutyric acid (GABA) production and genetic and phenotypic determinants.Microbial cell factories · 2023Article
- Influence of insertion sequences on population structure of phytopathogenic bacteria in theMicrobiology (Reading, England) · 2023Article
- From a large-scale genomic analysis of insertion sequences to insights into their regulatory roles in prokaryotes.BMC genomics · 2022Article
- A Novel Method for Long-Term Analysis of Lactic Acid and Ammonium Production in Non-growingFrontiers in bioengineering and biotechnology · 2020Article
- Genetics of Lactococci.Microbiology spectrum · 2019Review
- Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering.Materials today. Bio · 2019Article
- Random sequences rapidly evolve into de novo promoters.Nature communications · 2018Article
- Further Elucidation of Galactose Utilization inFrontiers in microbiology · 2018Article
- Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363.Applied and environmental microbiology · 2017Article
- Hyaluronan production and molecular weight is enhanced in pathway-engineered strains of lactate dehydrogenase-deficientMetabolic engineering communications · 2016Article
- Availability of public goods shapes the evolution of competing metabolic strategies.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Redox balance via lactate dehydrogenase is important for multiple stress resistance and virulence in Enterococcus faecalis.Infection and immunity · 2013Article
- Promoter propagation in prokaryotes.Nucleic acids research · 2012Article
- The putative lactococcal extracytoplasmic function anti-sigma factor llmg2447 determines resistance to the cell wall-active bacteriocin lcn972.Antimicrobial agents and chemotherapy · 2012Article
- Promoter reuse in prokaryotes.Mobile genetic elements · 2012Article
- A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363.Applied and environmental microbiology · 2012Article
- High yields of 2,3-butanediol and mannitol in Lactococcus lactis through engineering of NAD⁺ cofactor recycling.Applied and environmental microbiology · 2011Article
- Characterization of three lactic acid bacteria and their isogenic ldh deletion mutants shows optimization for YATP (cell mass produced per mole of ATP) at their physiological pHs.Applied and environmental microbiology · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactococcus lactis NZ9010 in which the las operon-encoded ldh gene was replaced with an erythromycin resistance gene cassette displayed a stable phenotype when grown under aerobic conditions, and its main end products of fermentation under these conditions were acetate and acetoin. However, under anaerobic conditions, the growth of these cells was strongly retarded while the main end products of fermentation were acetate and ethanol. Upon prolonged subculturing of this strain under anaerobic conditions, both the growth rate and the ability to produce lactate were recovered after a variable number of generations. This recovery was shown to be due to the transcriptional activation of a silent ldhB gene coding for an Ldh protein (LdhB) with kinetic parameters different from those of the native las operon-encoded Ldh protein. Nevertheless, cells producing LdhB produced mainly lactate as the end product of fermentation. The mechanism underlying the ldhB gene activation was primarily studied in a single-colony isolate of the recovered culture, designated L. lactis NZ9015. Integration of IS981 in the upstream region of ldhB was responsible for transcription activation of the ldhB gene by generating an IS981-derived -35 promoter region at the correct spacing with a natively present -10 region. Subsequently, analysis of 10 independently isolated lactate-producing derivatives of L. lactis NZ9010 confirmed that the ldhB gene is transcribed in all of them. Moreover, characterization of the upstream region of the ldhB gene in these derivatives indicated that site-specific and directional IS981 insertion represents the predominant mechanism of the observed recovery of the ability to produce lactate.
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