ArticleJournal of bacteriology2003
Expression of the secondary sigma factor sigmaX in Streptococcus pyogenes is restricted at two levels.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- The ComRS-SigX Pathway Regulates Natural Transformation in Streptococcus ferus.Journal of bacteriology · 2023Article
- The ComRS-SigX pathway regulates natural transformation inbioRxiv : the preprint server for biology · 2023Article
- Article
- Conserved and specific features of Streptococcus pyogenes and Streptococcus agalactiae transcriptional landscapes.BMC genomics · 2019Article
- Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.Applied and environmental microbiology · 2017Article
- Control of natural transformation in salivarius Streptococci through specific degradation of σX by the MecA-ClpCP protease complex.Journal of bacteriology · 2014Article
- The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone.Journal of bacteriology · 2012Article
- Adaptor protein MecA is a negative regulator of the expression of late competence genes in Streptococcus thermophilus.Journal of bacteriology · 2012Article
- Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus.PLoS pathogens · 2012Article
- Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW.Journal of bacteriology · 2009Article
- Streptococcus pyogenes CovRS mediates growth in iron starvation and in the presence of the human cationic antimicrobial peptide LL-37.Journal of bacteriology · 2009Article
- Shuttle expression plasmids for genetic studies in Streptococcus mutans.Microbiology (Reading, England) · 2008Article
- Involvement of sensor kinases in the stress tolerance response of Streptococcus mutans.Journal of bacteriology · 2008Article
- Regulation of gbpC expression in Streptococcus mutans.Journal of bacteriology · 2007Article
- CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus.Journal of bacteriology · 2007Article
- Natural genetic transformation: A novel tool for efficient genetic engineering of the dairy bacterium Streptococcus thermophilus.Applied and environmental microbiology · 2006Article
- The Rgg regulator of Streptococcus pyogenes influences utilization of nonglucose carbohydrates, prophage induction, and expression of the NAD-glycohydrolase virulence operon.Journal of bacteriology · 2006Article
- Spx is a global effector impacting stress tolerance and biofilm formation in Staphylococcus aureus.Journal of bacteriology · 2006Article
- Two distinct functions of ComW in stabilization and activation of the alternative sigma factor ComX in Streptococcus pneumoniae.Journal of bacteriology · 2005Article
- Rgg regulates growth phase-dependent expression of proteins associated with secondary metabolism and stress in Streptococcus pyogenes.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Secondary RNA polymerase sigma factors in many bacteria are responsible for regulating a vast range of processes including virulence. A protein (sigma(X)) in the gram-positive human pathogen Streptococcus pyogenes (the group A Streptococcus or GAS) was recently shown to function in vitro as a secondary sigma factor. We report here the isolation of a mutant in which both sigX genes are inactivated, show that sigma(X) functions in GAS cells, and show that the amount of sigma(X) is controlled at two levels. Primer extension analysis indicates that sigX transcription is low in GAS cells grown in Todd-Hewitt yeast broth, and immunoblot assays with a sigma(X)-specific polyclonal antibody demonstrate that the protein does not accumulate in these cells. To increase the level of sigX transcription in GAS, we constructed a strain that constitutively expresses the sigX gene from a heterologous promoter. Expression of sigX from this promoter led to transcription of the sigma(X)-dependent cinA promoter in GAS cells. We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of sigma(X) protein, which resulted in higher levels of transcription from the sigma(X)-dependent promoters cinA, smf, and cglA. In addition, a clpP mutant containing sigX only at its wild-type loci on the chromosome generated more transcription from the sigma(X)-dependent cinA promoter than did the wild-type parental strain. Therefore, sigma(X) activity in GAS is limited by low-level transcription of the sigX structural genes and by clpP, which appears to negatively regulate sigma(X) accumulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.