ArticleMolecular and cellular biology2003
Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 67 citations in OpenAlex.
- Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma Parasites.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Small-Molecule Inhibitors Targeting Proteasome-Associated Deubiquitinases.International journal of molecular sciences · 2021Review
- Adaptability of the ubiquitin-proteasome system to proteolytic and folding stressors.The Journal of cell biology · 2021Article
- Deubiquitination Reactions on the Proteasome for Proteasome Versatility.International journal of molecular sciences · 2020Review
- Inferring Gene Regulatory Networks from a Population of Yeast Segregants.Scientific reports · 2019Article
- Meddling with Fate: The Proteasomal Deubiquitinating Enzymes.Journal of molecular biology · 2017Review
- Hsp83 loss suppresses proteasomal activity resulting in an upregulation of caspase-dependent compensatory autophagy.Autophagy · 2017Article
- POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.Nature communications · 2015Article
- Ubiquitin-Mediated Regulation of Cell Death, Inflammation, and Defense of Homeostasis.Current topics in developmental biology · 2015Review
- Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth.Oncotarget · 2014Article
- Human cytomegalovirus UL76 elicits novel aggresome formation via interaction with S5a of the ubiquitin proteasome system.Journal of virology · 2013Article
- FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis.Nature communications · 2012Article
- ApoptoProteomics, an integrated database for analysis of proteomics data obtained from apoptotic cells.Molecular & cellular proteomics : MCP · 2012Article
- Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758).BMC genomics · 2011Article
- Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks.PloS one · 2010Article
- Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions.Genetics · 2010Article
- Polyubiquitin binding and disassembly by deubiquitinating enzymes.Chemical reviews · 2009Review
- Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.Biochemistry · 2009Article
- Disruption of Rpn4-induced proteasome expression in Saccharomyces cerevisiae reduces cell viability under stressed conditions.Genetics · 2008Article
- Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome.Molecular biology of the cell · 2008Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The S13 subunit (also called Pad1, Rpn11, and MPR1) is a component of the 19S complex, a regulatory complex essential for the ubiquitin-dependent proteolytic activity of the 26S proteasome. To address the functional role of S13, we combined double-stranded RNA interference (RNAi) against the Drosophila proteasome subunit DmS13 with expression of wild-type and mutant forms of the homologous human gene, HS13. These studies show that DmS13 is essential for 26S function. Loss of the S13 subunit in metazoan cells leads to increased levels of ubiquitin conjugates, cell cycle defects, DNA overreplication, and apoptosis. In vivo assays using short-lived proteasome substrates confirmed that the 26S ubiquitin-dependent degradation pathway is compromised in S13-depleted cells. In complementation experiments using Drosophila cell lines expressing HS13, wild-type HS13 was found to fully rescue the knockdown phenotype after DmS13 RNAi treatment, while an HS13 containing mutations (H113A-H115A) in the proposed isopeptidase active site was unable to rescue. A mutation within the conserved MPN/JAMM domain (C120A) abolished the ability of HS13 to rescue the Drosophila cells from apoptosis or DNA overreplication. However, the C120A mutant was found to partially restore normal levels of ubiquitin conjugates. The S13 subunit may possess multiple functions, including a deubiquitinylating activity and distinct activities essential for cell cycle progression that require the conserved C120 residue.
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