ArticleMolecular and cellular biology2003
Specificity and regulation of DNA binding by the yeast glucose transporter gene repressor Rgt1.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 130 citations in OpenAlex.
- Nonredundant roles for paralogous proteins in the yeast glucose-sensing pathway.Molecular biology of the cell · 2026Article
- Non-redundant roles for paralogous proteins in the yeast glucose-sensing pathway.bioRxiv : the preprint server for biology · 2025Article
- Integrative Transcriptomic and Target Metabolite Analysis as a New Tool for Designing Metabolic Engineering in Yeast.Biomolecules · 2024Article
- Effects of ScRgt1-Like DNA-binding transcription factor SpRgt1 (SPCC320.03) on Hexose transporters gene expression in Schizosaccharomyces pombe.Archives of microbiology · 2024Article
- Multiple roles for the cytoplasmic C-terminal domains of the yeast cell surface receptors Rgt2 and Snf3 in glucose sensing and signaling.Scientific reports · 2024Article
- Activity of the pleiotropic drug resistance transcription factors Pdr1p and Pdr3p is modulated by binding site flanking sequences.FEBS letters · 2024Article
- Glucose metabolism in the pathogenic free-living amoebae: Tempting targets for treatment development.Chemical biology & drug design · 2024Review
- Casein kinases are required for the stability of the glucose-sensing receptor Rgt2 in yeast.Scientific reports · 2022Article
- Multi-Omics Analysis of Multiple Glucose-Sensing Receptor Systems in Yeast.Biomolecules · 2022Article
- Unveiling the Role Displayed byJournal of fungi (Basel, Switzerland) · 2021Article
- Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS).Chemical reviews · 2021Article
- Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.Computational and structural biotechnology journal · 2021Review
- Promoter Architecture and Promoter Engineering inMetabolites · 2020Review
- MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus.Scientific reports · 2019Article
- Article
- Engineering of Pentose Transport inFrontiers in bioengineering and biotechnology · 2019Review
- Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor ofG3 (Bethesda, Md.) · 2018Article
- Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast.The Journal of biological chemistry · 2018Article
- Robustness of Nutrient Signaling Is Maintained by Interconnectivity Between Signal Transduction Pathways.Frontiers in physiology · 2018Article
- Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis.The Journal of cell biology · 2017Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rgt1 is a glucose-responsive transcription factor that binds to the promoters of several HXT genes encoding glucose transporters in Saccharomyces cerevisiae and regulates their expression in response to glucose. Rgt1 contains a Zn(2)Cys(6) binuclear cluster responsible for DNA binding. Most proteins that contain this sequence motif bind as dimers to regularly spaced pairs of the sequence CGG. However, there are no CGG pairs with regular spacing in promoters of genes regulated by Rgt1, suggesting that Rgt1 binds as a monomer to CGG or to another sequence. We identified the Rgt1 consensus binding site sequence 5'-CGGANNA-3', multiple copies of which are present in all HXT promoters regulated by Rgt1. Rgt1 binds in vivo to multiple sites in the HXT3 promoter in a nonadditive, synergistic manner, leading to synergistic repression of HXT3 transcription. We show that glucose inhibits the DNA-binding ability of Rgt1, thereby relieving repression of HXT gene expression. This regulation of Rgt1 DNA-binding activity is caused by its glucose-induced phosphorylation: the hyperphosphorylated Rgt1 present in cells growing on high levels of glucose does not bind DNA in vivo or in vitro; dephosphorylation of this form of Rgt1 in vitro restores its DNA-binding ability. Furthermore, an altered Rgt1 that functions as a constitutive repressor remains hypophosphorylated when glucose is added to cells and binds DNA under these conditions. These results suggest that glucose regulates the DNA-binding ability of Rgt1 by inducing its phosphorylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.