ArticleThe Journal of cell biology2003
Critical roles for the COOH-terminal NITY and RGT sequences of the integrin beta3 cytoplasmic domain in inside-out and outside-in signaling.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition model.Frontiers of medicine · 2016Article
- Review
- Article
- The interaction of Gα13 with integrin β1 mediates cell migration by dynamic regulation of RhoA.Molecular biology of the cell · 2015Article
- Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy.Journal of hematology & oncology · 2015Article
- Direct interaction of kindlin-3 with integrin αIIbβ3 in platelets is required for supporting arterial thrombosis in mice.Arteriosclerosis, thrombosis, and vascular biology · 2014Article
- Article
- Mitochondrially mediated integrin αIIbβ3 protein inactivation limits thrombus growth.The Journal of biological chemistry · 2013Article
- Spatial coordination of kindlin-2 with talin head domain in interaction with integrin β cytoplasmic tails.The Journal of biological chemistry · 2012Article
- Calpain-mediated proteolysis of paxillin negatively regulates focal adhesion dynamics and cell migration.The Journal of biological chemistry · 2011Article
- IP-10 induces dissociation of newly formed blood vessels.Journal of cell science · 2009Article
- Article
- Increased extracellular pressure enhances cancer cell integrin-binding affinity through phosphorylation of beta1-integrin at threonine 788/789.American journal of physiology. Cell physiology · 2009Article
- The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility.The Journal of biological chemistry · 2008Article
- Article
- Kindlin-2 controls bidirectional signaling of integrins.Genes & development · 2008Article
- Kindlin-2 (Mig-2): a co-activator of beta3 integrins.The Journal of cell biology · 2008Article
- A molecular switch that controls cell spreading and retraction.The Journal of cell biology · 2007Article
- Calpain is required for normal osteoclast function and is down-regulated by calcitonin.The Journal of biological chemistry · 2006Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bidirectional signaling of integrin alphaIIbbeta3 requires the beta3 cytoplasmic domain. To determine the sequence in the beta3 cytoplasmic domain that is critical to integrin signaling, cell lines were established that coexpress the platelet receptor for von Willebrand factor (vWF), glycoprotein Ib-IX, integrin alphaIIb, and mutants of beta3 with truncations at sites COOH terminal to T741, Y747, F754, and Y759. Truncation at Y759 did not affect integrin activation, as indicated by vWF-induced fibrinogen binding, but affected cell spreading and stable adhesion. Thus, the COOH-terminal RGT sequence of beta3 is important for outside-in signaling but not inside-out signaling. In contrast, truncation at F754, Y747, or T741 completely abolished integrin activation. A point mutation replacing Y759 with alanine also abolished integrin activation. Thus, the T755NITY759 sequence of beta3, containing an NXXY motif, is critical to inside-out signaling, whereas the intact COOH terminus is important for outside-in signaling. In addition, we found that the calcium-dependent protease calpain preferentially cleaves at Y759 in a population of beta3 during platelet aggregation and adhesion, suggesting that calpain may selectively regulate integrin outside-in signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.