ArticleProceedings of the National Academy of Sciences of the United States of America2003
Use of avian retroviral vectors to introduce transcriptional regulators into mammalian cells for analyses of tumor maintenance.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Differential bicistronic gene translation mediated by the internal ribosome entry site element of encephalomyocarditis virus.Biomedical journal · 2021Article
- Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system.Bioscience reports · 2019Article
- Fatty Acid Oxidation Mediated by Acyl-CoA Synthetase Long Chain 3 Is Required for Mutant KRAS Lung Tumorigenesis.Cell reports · 2016Article
- The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function.Nature communications · 2015Article
- A p53/ARF-dependent anticancer barrier activates senescence and blocks tumorigenesis without impacting apoptosis.Molecular cancer research : MCR · 2015Article
- Development of genetically flexible mouse models of sarcoma using RCAS-TVA mediated gene delivery.PloS one · 2014Article
- A novel TetR-regulating peptide turns off rtTA-mediated activation of gene expression.PloS one · 2014Article
- Depot formation of doxycycline impairs Tet-regulated gene expression in vivo.Transgenic research · 2012Article
- Translation-dependent mechanisms lead to PML upregulation and mediate oncogenic K-RAS-induced cellular senescence.EMBO molecular medicine · 2012Article
- Production of avian retroviruses and tissue-specific somatic retroviral gene transfer in vivo using the RCAS/TVA system.Nature protocols · 2012Article
- Targeted delivery of NRASQ61R and Cre-recombinase to post-natal melanocytes induces melanoma in Ink4a/Arflox/lox mice.Pigment cell & melanoma research · 2010Article
- Non-germline genetically engineered mouse models for translational cancer research.Nature reviews. Cancer · 2010Review
- Chimeric mouse tumor models reveal differences in pathway activation between ERBB family- and KRAS-dependent lung adenocarcinomas.Nature biotechnology · 2010Article
- IGFBP2 is a candidate biomarker for Ink4a-Arf status and a therapeutic target for high-grade gliomas.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Cell-specific transduction of Prdm1-expressing lineages mediated by a receptor for avian leukosis virus subgroup B.Journal of virology · 2009Article
- Signal transduction in transgenic mouse models of human breast cancer--implications for human breast cancer.Journal of mammary gland biology and neoplasia · 2008Review
- The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.Journal of virology · 2008Article
- A Cre-loxP-based mouse model for conditional somatic gene expression and knockdown in vivo by using avian retroviral vectors.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Lentivirus-mediated oncogene introduction into mammary cells in vivo induces tumors.Neoplasia (New York, N.Y.) · 2008Article
- Phospholipase D provides a survival signal in human cancer cells with activated H-Ras or K-Ras.Cancer letters · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A key issue in cancer biology is whether genetic lesions involved in tumor initiation or progression are required for tumor maintenance. This question can be addressed with mouse models that conditionally express oncogenic transgenes, i.e., under the control of tetracycline (tet)-dependent transcriptional regulators. We have developed a system for studying tumor maintenance by using avian retroviral [i.e., replication-competent avian leukosis virus long terminal repeat with splice acceptor (RCAS)] vectors to deliver the reverse tet transcriptional transactivator (rtTA) gene to somatic mammalian cells. rtTA can regulate any transgene in which the protein coding sequence is preceded by a tet-operator (tet-o); RCAS viruses infect only cells engineered to express ectopically the avian retroviral receptor, TVA. One vector, RCAS-rtTA-IRES-GFP, also encodes GFP to identify infected cells. Infection of cells from beta-actin TVA transgenic mice with this vector permits efficient regulation of tet-responsive transgenes. Sarcomas arise when p53-deficient murine embryonic fibroblasts carrying beta-actin TVA and tet-o-K-ras4bG12D transgenes are infected with RCAS-rtTA-IRES-GFP and introduced into nude mice treated with the tet analog, doxycycline (dox); when dox is withdrawn, K-ras4bG12D levels fall, cells undergo apoptosis, and tumors regress. Regression can be prevented by means of a genetic complementation assay in which tumors are superinfected before dox withdrawal with other RCAS viruses, such as those carrying an active allele of K-ras. Many TVA and tet-regulated transgenic mice have been generated; thus, this method for somatic cell-specific and temporally controlled gene expression may have broad applications for the study of oncogenesis and tumor maintenance, as well as other cell functions and development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.