ArticleMolecular biology of the cell2003
Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
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- Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanism.Nature communications · 2023Article
- TheFrontiers in cell and developmental biology · 2022Article
- Identification of Residues Controlling Restriction versus Enhancing Activities of IFITM Proteins on Entry of Human Coronaviruses.Journal of virology · 2018Article
- A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity.eLife · 2018Article
- CMTM7 knockdown increases tumorigenicity of human non-small cell lung cancer cells and EGFR-AKT signaling by reducing Rab5 activation.Oncotarget · 2015Article
- Bruton's tyrosine kinase mediates the synergistic signalling between TLR9 and the B cell receptor by regulating calcium and calmodulin.PloS one · 2013Article
- The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.Journal of virology · 2012Article
- Immune and inflammatory pathways are involved in inherent bone marrow ossification.Clinical orthopaedics and related research · 2012Article
- Article
- Tuberculosis: new aspects of an old disease.International journal of cell biology · 2011Article
- Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin.Molecular biology of the cell · 2008Article
- Perforin enhances the granulysin-induced lysis of Listeria innocua in human dendritic cells.BMC immunology · 2007Article
- The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Phosphorylation of EEA1 by p38 MAP kinase regulates mu opioid receptor endocytosis.The EMBO journal · 2005Article
- Protein kinaseCdelta-calmodulin crosstalk regulates epidermal growth factor receptor exit from early endosomes.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Ca2+ is an essential requirement in membrane fusion, acting through binding proteins such as calmodulin (CaM). Ca2+/CaM is required for early endosome fusion in vitro, however, the molecular basis for this requirement is unknown. An additional requirement for endosome fusion is the protein Early Endosome Antigen 1 (EEA1), and its recruitment to the endosome depends on phosphatidylinositol 3-phosphate [PI(3)P] and the Rab5 GTPase. Herein, we demonstrate that inhibition of Ca2+/CaM, by using either chemical inhibitors or specific antibodies directed to CaM, results in a profound inhibition of EEA1 binding to endosomal membranes both in live cells and in vitro. The concentration of Ca2+/CaM inhibitors required for a full dissociation of EEA1 from endosomal membranes had no effect on the activity of phosphatidylinositol 3-kinases or on endogenous levels of PI(3)P. However, the interaction of EEA1 with liposomes containing PI(3)P was decreased by Ca2+/CaM inhibitors. Thus, Ca2+/CaM seems to be required for the stable interaction of EEA1 with endosomal PI(3)P, perhaps by directly or indirectly stabilizing the quaternary organization of the C-terminal FYVE domain of EEA1. This requirement is likely to underlie at least in part the essential role of Ca2+/CaM in endosome fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.