ArticleMolecular biology of the cell2003
Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 214 citations in OpenAlex.
- The formation and function of intercellular bridges in male germlines.Communications biology · 2026Review
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Examination of Germline and Somatic Intercellular Bridges in Hydra vulgaris Reveals Insights into the Evolutionarily Conserved Mechanism of Intercellular Bridge Formation.Molecular biology and evolution · 2025Article
- Evidence against the role of toll-like receptors 7 and 8 in sex selection in mice, cattle, and humans.iScience · 2025Article
- Comparative transcriptome analysis of bull X- and Y-spermatozoa.Scientific reports · 2025Article
- Intercellular bridges are essential for transposon repression and meiosis in the male germline.Nature communications · 2025Article
- Spatiotemporal dynamics of spermatogenesis: insights from high-resolution spatial transcriptomics and pseudotime trajectories in mouse testes.Frontiers in reproductive health · 2025Article
- Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice.Journal of cellular and molecular medicine · 2024Article
- Investigation of the morphological and histological features of the testes of pigeon (Open veterinary journal · 2024Article
- Article
- Expression of Protein Markers in Spermatogenic and Supporting Sertoli Cells Affected by High Abdominal Temperature in Cryptorchidism Model Mice.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Non-Classical Intercellular Communications: Basic Mechanisms and Roles in Biology and Medicine.International journal of molecular sciences · 2023Review
- Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids.International journal of molecular sciences · 2022Article
- All for one: changes in mitochondrial morphology and activity during syncytial oogenesis†.Biology of reproduction · 2022Article
- Regulatory mechanisms of cytoneme-based morphogen transport.Cellular and molecular life sciences : CMLS · 2022Review
- Soma-to-germline RNA communication.Nature reviews. Genetics · 2022Review
- Uncoupling cell division and cytokinesis during germline development in metazoans.Frontiers in cell and developmental biology · 2022Review
- Communal living: the role of polyploidy and syncytia in tissue biology.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Subcellular localization of the mouse PRAMEL1 and PRAMEX1 reveals multifaceted roles in the nucleus and cytoplasm of germ cells during spermatogenesis.Cell & bioscience · 2021Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable cytoplasmic bridges (or ring canals) connecting the clone of spermatids are assumed to facilitate the sharing of haploid gene products and synchronous development of the cells. We have visualized these cytoplasmic bridges under phase-contrast optics and recorded the sharing of cytoplasmic material between the spermatids by a digital time-lapse imaging system ex vivo. A multitude of small (ca. 0.5 microm) granules were seen to move continuously over the bridges, but only 28% of those entering the bridge were actually transported into other cell. The average speed of the granules decreased significantly during the passage. Immunocytochemistry revealed that some of the shared granules contained haploid cell-specific gene product TRA54. We also demonstrate the novel function for the Golgi complex in acrosome system formation by showing that TRA54 is processed in Golgi complex and is transported into acrosome system of neighboring spermatid. In addition, we propose an intercellular transport function for the male germ cell-specific organelle chromatoid body. This mRNA containing organelle, ca. 1.8 microm in diameter, was demonstrated to go over the cytoplasmic bridge from one spermatid to another. Microtubule inhibitors prevented all organelle movements through the bridges and caused a disintegration of the chromatoid body. This is the first direct demonstration of an organelle traffic through cytoplasmic bridges in mammalian spermatogenesis. Golgi-derived haploid gene products are shared between spermatids, and an active involvement of the chromatoid body in intercellular material transport between round spermatids is proposed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.