ArticlePlant physiology2003
Identification and differential expression of two thioredoxin h isoforms in germinating seeds from pea.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- GWAS and transcriptome analysis reveal MADS26 involved in seed germination ability in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- The expression pattern of OsDim1 in rice and its proposed function.Scientific reports · 2019Article
- Simultaneous Expression of GUS and Actin Genes by Using the Multiplex RT-PCR and Multiplex Gold Nanoparticle Probes.Journal of fluorescence · 2018Article
- 5' to 3' mRNA Decay Contributes to the Regulation of Arabidopsis Seed Germination by Dormancy.Plant physiology · 2017Article
- Mitochondrial AtTrxo1 is transcriptionally regulated by AtbZIP9 and AtAZF2 and affects seed germination under saline conditions.Journal of experimental botany · 2017Article
- Article
- Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat.Scientific reports · 2016Article
- Beneficial Roles of Melatonin on Redox Regulation of Photosynthetic Electron Transport and Synthesis of D1 Protein in Tomato Seedlings under Salt Stress.Frontiers in plant science · 2016Article
- Germination Potential of Dormant and Nondormant Arabidopsis Seeds Is Driven by Distinct Recruitment of Messenger RNAs to Polysomes.Plant physiology · 2015Article
- Article
- Expression of the chloroplast thioredoxins f and m is linked to short-term changes in the sugar and thiol status in leaves of Pisum sativum.Journal of experimental botany · 2012Article
- Isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup III of h-type Trxs from grape (Vitis vinifera L. cv. Askari).Molecular biology reports · 2012Article
- Identification and characterization of thioredoxin h isoforms differentially expressed in germinating seeds of the model legume Medicago truncatula.Plant physiology · 2011Article
- Circadian regulation of chloroplastic f and m thioredoxins through control of the CCA1 transcription factor.Journal of experimental botany · 2011Article
- The conformational stability and biophysical properties of the eukaryotic thioredoxins of Pisum sativum are not family-conserved.PloS one · 2011Article
- Identification of changes in wheat (Triticum aestivum L.) seeds proteome in response to anti-trx s gene.PloS one · 2011Article
- A novel type of thioredoxin dedicated to symbiosis in legumes.Plant physiology · 2008Article
- Sequence and expression analysis of the thioredoxin protein gene family in rice.Molecular genetics and genomics : MGG · 2008Article
- The NADPH-dependent thioredoxin reductase/thioredoxin system in germinating barley seeds: gene expression, protein profiles, and interactions between isoforms of thioredoxin h and thioredoxin reductase.Plant physiology · 2008Article
- Localization in roots and flowers of pea chloroplastic thioredoxin f and thioredoxin m proteins reveals new roles in nonphotosynthetic organs.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NADPH/NADP-thioredoxin (Trx) reductase (NTR)/Trx system (NTS) is a redox system that plays a posttranslational regulatory role by reducing protein targets involved in crucial cellular processes in microorganisms and animals. In plants, the system includes several h type Trx isoforms and has been shown to intervene in reserve mobilization during early seedling growth of cereals. To determine whether NTS was operational during germination of legume seeds and which Trx h isoforms could be implicated, Trx h isoforms expression was monitored in germinating pea (Pisum sativum cv Baccara) seeds, together with the amount of NTR and NADPH. Two new isoforms were identified: Trx h3, similar to the two isoforms already described in pea but not expressed in seeds; and the more divergent isoform, Trx h4. Active recombinant proteins were produced in Escherichia coli and used to raise specific antibodies. The expression of new isoforms was analyzed at both mRNA and protein levels. The lack of correlation between mRNA and protein abundances suggests the occurrence of posttranscriptional regulation. Trx h3 protein amount remained constant in both axes and cotyledons of dry and imbibed seeds but then decreased 2 d after radicle protrusion. In contrast, Trx h4 was only expressed in axes of dry and imbibed seeds but not in germinated seeds or in seedlings, therefore appearing as closely linked to germination. The presence of NTR and NADPH in seeds suggests that NTS could be functional during germination. The possible role of Trx h3 and h4 in this context is discussed.
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