ArticlePlant physiology2003
Expression of anthocyanins and proanthocyanidins after transformation of alfalfa with maize Lc.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 122 citations in OpenAlex.
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- A Comprehensive Review of Phenolic Compounds in Horticultural Plants.International journal of molecular sciences · 2025Review
- The Lotus corniculatus MYB5 functions as a master regulator in proanthocyanidin biosynthesis and bioengineering.Plant cell reports · 2024Article
- Evolutionary trajectory of transcription factors and selection of targets for metabolic engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- An unconventional proanthocyanidin pathway in maize.Nature communications · 2023Article
- Anthocyanin metabolism in Nelumbo: translational and post-translational regulation control transcription.BMC plant biology · 2023Article
- Enhancement of the anthocyanin contents ofFrontiers in plant science · 2023Article
- Comparative Analysis of Proanthocyanidin Metabolism and Genes Regulatory Network in Fresh Leaves of Two Different Ecotypes ofPlants (Basel, Switzerland) · 2022Article
- HybridFrontiers in veterinary science · 2022Article
- Article
- Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots OverexpressingInternational journal of molecular sciences · 2021Article
- Article
- Differential Production of Phenolics, Lipids, Carbohydrates and Proteins in Stressed and Unstressed Aquatic Plants,Biology · 2020Article
- The interplay between miR156/SPL13 and DFR/WD40-1 regulate drought tolerance in alfalfa.BMC plant biology · 2019Article
- Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa).PloS one · 2019Article
- Gene-Transformation-Induced Changes in Chemical Functional Group Features and Molecular Structure Conformation in Alfalfa Plants Co-Expressing Lc-bHLH and C1-MYB Transcriptive Flavanoid Regulatory Genes: Effects of Single-Gene and Two-Gene Insertion.International journal of molecular sciences · 2017Article
- The Use of Gene Modification and Advanced Molecular Structure Analyses towards Improving Alfalfa Forage.International journal of molecular sciences · 2017Review
- Comparative transcriptome sequencing and de novo analysis of Vaccinium corymbosum during fruit and color development.BMC plant biology · 2016Article
- The Role of Proanthocyanidins Complex in Structure and Nutrition Interaction in Alfalfa Forage.International journal of molecular sciences · 2016Review
- Multigene synergism increases the isoflavone and proanthocyanidin contents of Medicago truncatula.Plant biotechnology journal · 2016Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three anthocyanin regulatory genes of maize (Zea mays; Lc, B-Peru, and C1) were introduced into alfalfa (Medicago sativa) in a strategy designed to stimulate the flavonoid pathway and alter the composition of flavonoids produced in forage. Lc constructs included a full-length gene and a gene with a shortened 5'-untranslated region. Lc RNA was strongly expressed in Lc transgenic alfalfa foliage, but accumulation of red-purple anthocyanin was observed only under conditions of high light intensity or low temperature. These stress conditions induced chalcone synthase and flavanone 3-hydroxylase expression in Lc transgenic alfalfa foliage compared with non-transformed plants. Genotypes containing the Lc transgene construct with a full-length 5'-untranslated region responded more quickly to stress conditions and with a more extreme phenotype. High-performance liquid chromatography analysis of field-grown tissue indicated that flavone content was reduced in forage of the Lc transgenic plants. Leucocyanidin reductase, the enzyme that controls entry of metabolites into the proanthocyanidin pathway, was activated both in foliage and in developing seeds of the Lc transgenic alfalfa genotypes. Proanthocyanidin polymer was accumulated in the forage, but (+)-catechin monomers were not detected. B-Peru transgenic and C1 transgenic populations displayed no visible phenotypic changes, although these transgenes were expressed at detectable levels. These results support the emerging picture of Lc transgene-specific patterns of expression in different recipient species. These results demonstrate that proanthocyanidin biosynthesis can be stimulated in alfalfa forage using an myc-like transgene, and they pave the way for the development of high quality, bloat-safe cultivars with ruminal protein bypass.
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