ArticlePlant molecular biology2003
Complete sequence analysis of transgene loci from plants transformed via microprojectile bombardment.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 87 citations in OpenAlex.
- A perspective from the EU: unintended genetic changes in plants caused by NGT-their relevance for a comprehensive molecular characterisation and risk assessment.Frontiers in bioengineering and biotechnology · 2023Review
- Identification and characterization of Sr22b, a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group.Plant biotechnology journal · 2022Article
- Deficiencies in the Risk Assessment of Genetically Engineered Bt Cowpea Approved for Cultivation in Nigeria: A Critical Review.Plants (Basel, Switzerland) · 2022Review
- The Generic Risks and the Potential of SDN-1 Applications in Crop Plants.Plants (Basel, Switzerland) · 2021Review
- Article
- Spatiotemporal Controllability and Environmental Risk Assessment of Genetically Engineered Gene Drive Organisms from the Perspective of European Union Genetically Modified Organism Regulation.Integrated environmental assessment and management · 2020Review
- Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.BMC biotechnology · 2020Article
- Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize.The Plant cell · 2019Article
- Effect of gene order in DNA constructs on gene expression upon integration into plant genome.3 Biotech · 2017Article
- Plant artificial chromosome technology and its potential application in genetic engineering.Plant biotechnology journal · 2016Review
- A comparative analysis of insertional effects in genetically engineered plants: considerations for pre-market assessments.Transgenic research · 2015Review
- Transgenic maize event TC1507: Global status of food, feed, and environmental safety.GM crops & food · 2015Review
- Improved molecular tools for sugar cane biotechnology.Plant molecular biology · 2014Article
- Nonhomologous end joining-mediated gene replacement in plant cells.Plant physiology · 2013Article
- Formation of complex extrachromosomal T-DNA structures in Agrobacterium tumefaciens-infected plants.Plant physiology · 2012Article
- Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco.Genetics and molecular biology · 2012Article
- Artificial chromosome formation in maize (Zea mays L.).Chromosoma · 2009Article
- Variable T-DNA linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice.Plant cell reports · 2007Article
- Transgene constructs in coho salmon (Oncorhynchus kisutch) are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite DNA.Transgenic research · 2006Article
- The mutational consequences of plant transformation.Journal of biomedicine & biotechnology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A substantial literature exists characterizing transgene locus structure from plants transformed via Agrobacterium and direct DNA delivery. However, there is little comprehensive sequence analysis of transgene loci available, especially from plants transformed by direct delivery methods. The goal of this study was to completely sequence transgene loci from two oat lines transformed via microprojectile bombardment that were shown to have simple transgene loci by Southern analysis. In line 3830, transformed with a single plasmid, one major and one of two minor loci were completely sequenced. Both loci exhibited rearranged delivered DNA and flanking genomic sequences. The minor locus contained only 296 bp of two non-contiguous fragments of the delivered DNA flanked by genomic (filler) DNA that did not originate from the integration target site. Predicted recognition sites for topoisomerase II and a MAR region were observed in the transgene integration target site for this non-functional minor locus. Line 11929, co-transformed with two different plasmids, had a single relatively simple transgene locus composed of truncated and rearranged sequences from both delivered DNAs. The transgene loci in both lines exhibited multiple transgene and genomic DNA rearrangements and regions of scrambling characteristic of complex transgene loci. The similar characteristics of recombined fragments and junctions in both transgenic oat lines implicate similar mechanisms of transgene integration and rearrangement regardless of the number of co-transformed plasmids and the level of transgene locus complexity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.