ArticleBMC genetics2003
Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids.
Article in BMC genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Evolution of Reproductive Division of Labor - Lessons Learned From the Social AmoebaFrontiers in cell and developmental biology · 2021Article
- The fate of cells undergoing spontaneous DNA damage during development.Development (Cambridge, England) · 2019Article
- Function of small GTPases in Dictyostelium macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection.PLoS pathogens · 2019Article
- Review
- Homeodomain-like DNA binding proteins control the haploid-to-diploid transition inScience advances · 2017Article
- WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A deep coverage Dictyostelium discoideum genomic DNA library replicates stably in Escherichia coli.Genomics · 2015Article
- Pharmacogenetics of resistance to Cisplatin and other anticancer drugs and the role of sphingolipid metabolism.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics.BMC genetics · 2007Article
- Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs.EMBO reports · 2007Article
- The dictyostelium kinome--analysis of the protein kinases from a simple model organism.PLoS genetics · 2006Article
- The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles.Genome biology · 2005Article
- A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.Nucleic acids research · 2004Article
- Aberrant spindle dynamics and cytokinesis in Dictyostelium discoideum cells that lack glycogen synthase kinase 3.European journal of cell biologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe relative ease of targeted gene disruption in the social amoeba Dictyostelium has stimulated its widespread use as an experimental organism for cell and developmental biology. However, the field has been hamstrung by the lack of techniques to recombine disrupted genes.
resultsWe describe new techniques for parasexual fusion of strains in liquid medium, selection and maintenance of the resulting stable diploid strains, and segregation to make recombined haploids. We have used these techniques to isolate rasS/gefB double nulls. The phenotypes of these mutants are no more severe than either parent, with movement, phagocytosis and fluid-phase endocytosis affected to the same degree as in rasS or gefB single nulls. In addition, we have produced diploids from one AX2- and one AX3-derived parent, providing an axenic strain with fewer secondary phenotypes than has been previously available.
conclusionsThe phenotype of the rasS/gefB double mutant suggests that the RasS and GefB proteins lie on the same linear pathway. In addition, axenic diploids and the techniques to generate, maintain and segregate them will be productive tools for future work on Dictyostelium. They will particularly facilitate generation of multiple mutants and manipulation of essential genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.