ArticleNucleic acids research2003
On the number of protein-protein interactions in the yeast proteome.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- 800 million years of co-evolution in the green plant lineage: the case of LEUNIG and SEUSS transcriptional co-regulators.Molecular biology and evolution · 2026Article
- Experimental assessment of AI-based interactome mapping.Nature communications · 2026Article
- Cell-cell communication: new insights and clinical implications.Signal transduction and targeted therapy · 2024Review
- CombFold: predicting structures of large protein assemblies using a combinatorial assembly algorithm and AlphaFold2.Nature methods · 2024Article
- Predicting structures of large protein assemblies using combinatorial assembly algorithm and AlphaFold2.bioRxiv : the preprint server for biology · 2023Article
- Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Convergent and distributed effects of the 3q29 deletion on the human neural transcriptome.Translational psychiatry · 2021Article
- BIPSPI: a method for the prediction of partner-specific protein-protein interfaces.Bioinformatics (Oxford, England) · 2019Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Article
- Scoring Large-Scale Affinity Purification Mass Spectrometry Datasets with MiST.Current protocols in bioinformatics · 2015Article
- Exploring bacterial organelle interactomes: a model of the protein-protein interaction network in the Pdu microcompartment.PLoS computational biology · 2015Article
- Multi-level machine learning prediction of protein-protein interactions in Saccharomyces cerevisiae.PeerJ · 2015Article
- Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.Cellular and molecular life sciences : CMLS · 2014Review
- The origins of the evolutionary signal used to predict protein-protein interactions.BMC evolutionary biology · 2012Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Article
- Binding site prediction for protein-protein interactions and novel motif discovery using re-occurring polypeptide sequences.BMC bioinformatics · 2011Article
- Aging and chronic DNA damage response activate a regulatory pathway involving miR-29 and p53.The EMBO journal · 2011Article
- Assembling a protein-protein interaction map of the SSU processome from existing datasets.PloS one · 2011Article
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1 author.
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Abstract
Using two different approaches, we estimated that on average there are about five interacting partners per protein in the proteome of the yeast Saccharomyces cerevisiae. In the first approach, we used a novel method to model sampling overlap by a Bernoulli process, compared the results of two independent yeast two-hybrid interaction screens and tested the robustness of the estimate. The most stable estimate of five interactors per protein was obtained when the three most highly connected nodes in the protein interaction network were removed from the analysis (eight interactors per protein if those nodes were kept). In the second approach, we analysed a published high-confidence subset of putative interaction data obtained from multiple sources, including large-scale two-hybrid screens, complex purifications, synthetic lethals, correlated gene expression, computational predictions and previous annotations. Strikingly, the estimate was again five interactors per protein. These estimates suggest a range of approximately 16,000-26,000 different interaction pairs in the yeast, excluding homotypic interactions. We also discuss the approaches to estimating the rate of homotypic interactions.
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