ArticleNucleic acids research2003
GAGA facilitates binding of Pleiohomeotic to a chromatinized Polycomb response element.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 71 citations in OpenAlex.
- Looping specificity of Polycomb response elements requires GAF and a combination of looping factors that could form a code.Nucleic acids research · 2026Article
- Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- Comparative transcriptome analyses of the Drosophila pupal eye.G3 (Bethesda, Md.) · 2021Article
- The bithorax complex iab-7 Polycomb response element has a novel role in the functioning of the Fab-7 chromatin boundary.PLoS genetics · 2018Article
- Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness inDevelopment (Cambridge, England) · 2018Article
- Global changes of H3K27me3 domains and Polycomb group protein distribution in the absence of recruiters Spps or Pho.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Vertebrate GAF/ThPOK: emerging functions in chromatin architecture and transcriptional regulation.Cellular and molecular life sciences : CMLS · 2018Review
- Sex combs reduced (Scr) regulatory region of Drosophila revisited.Molecular genetics and genomics : MGG · 2017Article
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Atrophin controls developmental signaling pathways via interactions with Trithorax-like.eLife · 2017Article
- Article
- New insights for Drosophila GAGA factor in larvae.Royal Society open science · 2015Article
- High-resolution mapping defines the cooperative architecture of Polycomb response elements.Genome research · 2014Article
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- Mapping polycomb response elements at the Drosophilla melanogaster giant locus.G3 (Bethesda, Md.) · 2013Article
- Polycomb silencing of the Drosophila 4E-BP gene regulates imaginal disc cell growth.Developmental biology · 2013Article
- Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.PloS one · 2013Article
- Polycomb group response elements in Drosophila and vertebrates.Advances in genetics · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polycomb response elements (PREs) are chromosomal elements, typically comprising thousands of base pairs of poorly defined sequences that confer the maintenance of gene expression patterns by Polycomb group (PcG) repressors and trithorax group (trxG) activators. Genetic studies have indicated a synergistic requirement for the trxG protein GAGA and the PcG protein Pleiohomeotic (PHO) in silencing at several PREs. However, the molecular basis of this cooperation remains unknown. Here, using DNaseI footprinting analysis, we provide a high-resolution map of sites for the sequence- specific DNA-binding PcG protein PHO, trxG proteins GAGA and Zeste and the gap protein Hunchback (HB) on the 1.6 kb Ultrabithorax (Ubx) PRE. Although these binding elements are present throughout the PRE, they display clear patterns of clustering, suggestive of functional collaboration at the level of PRE binding. We found that while GAGA could efficiently bind to a chromatinized PRE, PHO alone was incapable of binding to chromatin. However, PHO binding to chromatin, but not naked DNA, was strongly facilitated by GAGA, indicating interdependence between GAGA and PHO already at the level of PRE binding. These results provide a biochemical explanation for the in vivo cooperation between GAGA and PHO and suggest that PRE function involves the integrated activities of genetically antagonistic trxG and PcG proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.