ArticleNucleic acids research2003
Concerted folding of a Candida ribozyme into the catalytically active structure posterior to a rapid RNA compaction.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation.Genome biology · 2020Article
- Effects of Preferential Counterion Interactions on the Specificity of RNA Folding.The journal of physical chemistry letters · 2018Article
- Applications of synchrotron-based spectroscopic techniques in studying nucleic acids and nucleic acid-functionalized nanomaterials.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- Chain length determines the folding rates of RNA.Biophysical journal · 2012Article
- Slow formation of a pseudoknot structure is rate limiting in the productive co-transcriptional folding of the self-splicing Candida intron.RNA (New York, N.Y.) · 2009Article
- A phage RNA-binding protein binds to a non-cognate structured RNA and stabilizes its core structure.Biochemical and biophysical research communications · 2009Article
- Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent omegaG binding to a group I intron.Nucleic acids research · 2008Article
- Pentamidine binds to tRNA through non-specific hydrophobic interactions and inhibits aminoacylation and translation.Nucleic acids research · 2008Article
- Tertiary interactions determine the accuracy of RNA folding.Journal of the American Chemical Society · 2008Article
- GISSD: Group I Intron Sequence and Structure Database.Nucleic acids research · 2008Article
- Monovalent cations use multiple mechanisms to resolve ribozyme misfolding.RNA (New York, N.Y.) · 2006Article
- Fast formation of the P3-P7 pseudoknot: a strategy for efficient folding of the catalytically active ribozyme.RNA (New York, N.Y.) · 2005Article
- A peripheral element assembles the compact core structure essential for group I intron self-splicing.Nucleic acids research · 2005Article
- Predicting the secondary structures and tertiary interactions of 211 group I introns in IE subgroup.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Folding of the major population of Tetrahymena intron RNA into the catalytically active structure is trapped in a slow pathway. In this report, folding of Candida albicans intron was investigated using the trans-acting Ca.L-11 ribozyme as a model. We demonstrated that both the catalytic activity (k(obs)) and compact folding equilibrium of Ca.L-11 are strongly dependent on Mg(2+) at physiological concentrations, with both showing an Mg(2+) Hill coefficient of 3. Formation of the compact structure of Ca.L-11 is shown to occur very rapidly, on a subsecond time scale similar to that of RNase T1 cleavage. Most of the ribozyme RNA population folds into the catalytically active structure with a rate constant of 2 min(-1) at 10 mM Mg(2+); neither slower kinetics nor obvious Mg(2+) inhibition is observed. These results suggest that folding of the Ca.L-11 ribozyme is initiated by a rapid magnesium-dependent RNA compaction, which is followed by a slower searching for the native contacts to form the catalytically active structure without interference from the long-lived trapped states. This model thus provides an ideal system to address a range of interesting aspects of RNA folding, such as conformational searching, ion binding and the role of productive intermediates.
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