ArticleClinical and diagnostic laboratory immunology2003
Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- To establish an indirect ELISA method for the detection of human brucellosis based on signal peptide-truncated recombinant Cu/Zn SOD and its preliminary clinical verification.Frontiers in cellular and infection microbiology · 2026Article
- Multiepitope fusion protein-based ELISA for enhanced brucellosis serodiagnosis.PLoS neglected tropical diseases · 2025Article
- Development and evaluation of multiepitope fusion proteins for serological diagnosis of animal brucellosis.Microbiology spectrum · 2025Article
- Follow-up of antibody changes in brucellosis patients in Gansu, China.Microbiology spectrum · 2025Article
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
- Baculovirus-Assisted Production ofPathogens (Basel, Switzerland) · 2024Article
- Production and evaluation of anti-BP26 monoclonal antibodies for the serological detection of animal brucellosis.Frontiers in veterinary science · 2024Article
- Brucellosis detection and the role ofVeterinary world · 2023Review
- Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques.BMC veterinary research · 2022Article
- Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview.Pathogens (Basel, Switzerland) · 2021Review
- Comparative evaluation of the immunodominant proteins ofVeterinary world · 2021Article
- A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis.BMC veterinary research · 2021Article
- Adhesins ofPathogens (Basel, Switzerland) · 2020Review
- Diagnosis of human and canineHeliyon · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Binding of Brucella protein, Bp26, to select extracellular matrix molecules.BMC molecular and cell biology · 2019Article
- Characterization of Cell Envelope Multiple Mutants ofFrontiers in microbiology · 2018Article
- Immunoreactivity evaluation of a new recombinant chimeric protein againstIranian journal of microbiology · 2016Article
- Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species.Open veterinary journal · 2016Article
- Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay.BMC veterinary research · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes of the seven B. abortus biovar reference strains and B. abortus S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the bp26 nucleotide sequence for B. abortus S19 was almost identical to that found for B. melitensis 16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same B. abortus strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of B. melitensis and B. ovis infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from B. melitensis- or B. ovis-infected sheep while it avoided false-positive reactions observed with sera from Brucella-free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep.
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