ArticleBMC cell biology2003
Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology.
Article in BMC cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Tracing MYC Expression for Small Molecule Discovery.Cell chemical biology · 2019Article
- Control of cancer formation by intrinsic genetic noise and microenvironmental cues.Nature reviews. Cancer · 2015Review
- New aspects in the synthesis and secretion of lysozyme by cultured human monocyte cell lines.In vitro cellular & developmental biology. Animal · 2010Article
- Post-transcriptional gene regulation: from genome-wide studies to principles.Cellular and molecular life sciences : CMLS · 2008Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundInteractions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner.
resultsUsing this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype.
conclusionsWe conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.
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