ArticleProceedings of the National Academy of Sciences of the United States of America2003
Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 264 citations in OpenAlex.
- Trial
- Trial
- Advanced Dental Composite Technology via Bisilanized Dual-Action Nanofillers for Biofilm Control.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Genomic characterization ofApplied and environmental microbiology · 2026Article
- Direct-fed Microbials (DFM) and Poultry Genomics: A Synergistic Approach to Sustainable Antibiotic Free Farming.Probiotics and antimicrobial proteins · 2026Review
- Fructooligosaccharides-driven metabolism of a definedFrontiers in microbiology · 2026Article
- The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution.BMC genomics · 2025Article
- Classifying compounds as prebiotics - scientific perspectives and recommendations.Nature reviews. Gastroenterology & hepatology · 2025Review
- Advancements in the Heterologous Expression of Sucrose Phosphorylase and Its Molecular Modification for the Synthesis of Glycosylated Products.Molecules (Basel, Switzerland) · 2024Review
- Lacticaseibacillus paracasei completely utilizes fructooligosacchrides in the human gut through β-fructosidase (FosE).World journal of microbiology & biotechnology · 2024Article
- Freshwater microalgae Nannochloropsis limnetica for the production of β-galactosidase from whey powder.Scientific reports · 2024Article
- Screening Microbial Interactions During Inulin Utilization Reveals Strong Competition and Proteomic Changes in Lacticaseibacillus paracasei M38.Probiotics and antimicrobial proteins · 2024Article
- Innovative Approaches for Maintaining and Enhancing Skin Health and Managing Skin Diseases through Microbiome-Targeted Strategies.Antibiotics (Basel, Switzerland) · 2023Review
- Screening of Bifidobacteria with Probiotic Potential from Healthy Infant Feces by Using 2'-Fucosyllactose.Foods (Basel, Switzerland) · 2023Article
- Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting.Nature communications · 2023Article
- Lactobacilli and human dental caries: more than mechanical retention.Microbiology (Reading, England) · 2022Article
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.International journal of molecular sciences · 2022Review
- Mango Pectic Oligosaccharides: A Novel Prebiotic for Functional Food.Frontiers in nutrition · 2022Review
- An Integrative Multiomics Approach to Characterize Prebiotic Inulin Effects onFrontiers in bioengineering and biotechnology · 2022Article
- Cassava Starch as an Effective Texture Corrector of Fat-Free Dairy Products Based on Symbiotic Starter Culture.International journal of food science · 2022Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the LacI family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre-like sequences identified in the promoter-operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae. However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus. This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.