ArticleThe Journal of cell biology2003
Hrs regulates early endosome fusion by inhibiting formation of an endosomal SNARE complex.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 96 citations in OpenAlex.
- Pulmonary mitochondrial DNA release and activation of the cGAS-STING pathway in Lethal Stx12 knockout mice.Cell communication and signaling : CCS · 2025Article
- The clues offered by SNAREs on the vacuoles of plants and animals.Frontiers in plant science · 2025Review
- Drosophila Evi5 is a critical regulator of intracellular iron transport via transferrin and ferritin interactions.Nature communications · 2024Article
- Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic.Nature communications · 2024Article
- The crustacean DNA virus tegument protein VP26 binds to SNAP29 to inhibit SNARE complex assembly and autophagic degradation.Journal of virology · 2024Article
- Transport mechanisms between the endocytic, recycling, and biosynthetic pathways via endosomes and theFrontiers in cell and developmental biology · 2024Review
- UBE4B interacts with the ITCH E3 ubiquitin ligase to induce Ku70 and c-FLIPL polyubiquitination and enhanced neuroblastoma apoptosis.Cell death & disease · 2023Article
- Diminished Neuronal ESCRT-0 Function Exacerbates AMPA Receptor Derangement and Accelerates Prion-Induced Neurodegeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- An Overview of the Dry Eye Disease in Sjögren's Syndrome Using Our Current Molecular Understanding.International journal of molecular sciences · 2023Review
- Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation.Life science alliance · 2022Article
- The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.Molecular and cellular neurosciences · 2020Article
- PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.American journal of human genetics · 2017Article
- Oxidized phagosomal NOX2 complex is replenished from lysosomes.Journal of cell science · 2017Article
- STX13 regulates cargo delivery from recycling endosomes during melanosome biogenesis.Journal of cell science · 2015Article
- Motor and Sensory Deficits in the teetering Mice Result from Mutation of the ESCRT Component HGS.PLoS genetics · 2015Article
- UBE4B protein couples ubiquitination and sorting machineries to enable epidermal growth factor receptor (EGFR) degradation.The Journal of biological chemistry · 2014Article
- Ca²⁺-sensor proteins in the autophagic and endocytic traffic.Current protein & peptide science · 2013Review
- Article
- Where EGF receptors transmit their signals.Science signaling · 2012Article
- The phosphoinositide-associated protein Rush hour regulates endosomal trafficking in Drosophila.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Movement through the endocytic pathway occurs principally via a series of membrane fusion and fission reactions that allow sorting of molecules to be recycled from those to be degraded. Endosome fusion is dependent on SNARE proteins, although the nature of the proteins involved and their regulation has not been fully elucidated. We found that the endosome-associated hepatocyte responsive serum phosphoprotein (Hrs) inhibited the homotypic fusion of early endosomes. A region of Hrs predicted to form a coiled coil required for binding the Q-SNARE, SNAP-25, mimicked the inhibition of endosome fusion produced by full-length Hrs, and was sufficient for endosome binding. SNAP-25, syntaxin 13, and VAMP2 were bound from rat brain membranes to the Hrs coiled-coil domain. Syntaxin 13 inhibited early endosomal fusion and botulinum toxin/E inhibition of early endosomal fusion was reversed by addition of SNAP-25(150-206), confirming a role for syntaxin 13, and establishing a role for SNAP-25 in endosomal fusion. Hrs inhibited formation of the syntaxin 13-SNAP-25-VAMP2 complex by displacing VAMP2 from the complex. These data suggest that SNAP-25 is a receptor for Hrs on early endosomal membranes and that the binding of Hrs to SNAP-25 on endosomal membranes inhibits formation of a SNARE complex required for homotypic endosome fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.